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FFA2 (non-phospho-FFA2 Antibody)

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  • 7TM0226N
  • 100 µl
  • Rabbit
The non-phospho-FFA2 receptor antibody is directed against the distal part of the... more

The non-phospho-FFA2 receptor antibody is directed against the distal part of the carboxyl-terminal tail of human FFA2. It can be used to detect total FFA2 receptors in Western blots independent of phosphorylation. The non-phospho-FFA2 antibody can also be used to isolate and enrich FFA2 receptors from cell and tissue lysates. It also detects FFA2 in cultured cells and tissue sections by immunohistochemistry.

  Alternative Names FFAR2 IUPHAR Target ID   226... more

 

Alternative Names FFAR2
IUPHAR Target ID  226
UniProt ID  O15552
Western Blot (WB)  1:1000
Immunhistochemistry (IHC) 1:100
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic peptide presents the carboxyl-terminal tail of human FFA2.
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Validation of the FFA2 in transfected HEK293 cells.  Flp-In TREx HEK293 cells... more

Figure 1. Validation of the FFA2 in transfected HEK293 cells. Flp-In TREx HEK293 cells were induced to express human FFA2 DREADD-eYFP were  treated with vehicle or 100 nM sorbate for 5 min. FFA2 receptors were enriched using a GFP tra and immunoblotted with the phosphorylation-independent anti-FFA2 antibody (7TM0226N-IC) at a dilution of 1:1000.

Figure 2. Immunohistochemical identification of FFA2 Receptor in pancreas. Sections were dewaxed, microwaved in citric acid, and incubated with anti-FFA2 (FFA2 Receptor) antibody (7TM0226N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, FFA2 receptors were detected in a subset of pacreatic islet cells and at th eplasma membrane of exocrine pancreas cells.

Figure 3. Immunohistochemical identification of FFA2 Receptor in liver. Sections were dewaxed, microwaved in citric acid, and incubated with anti-FFA2 (FFA2 Receptor) antibody (7TM0226N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, FFA2 receptors were detected at the plasma membrane of hepatocytes.


Figure 4. Immunohistochemical identification of FFA2 Receptor in colon carcinoma. Sections were dewaxed, microwaved in citric acid, and incubated with anti-FFA2 (FFA2 Receptor) antibody (7TM0226N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, FFA2 receptors were detected at the plasma membrane of colon carcinoma cells.

Barki N, Jenkins L, Marsango S, Dedeo D, Bolognini D, Dwomoh L, Abdelmalik AM, Nilsen M,... more

Barki N, Jenkins L, Marsango S, Dedeo D, Bolognini D, Dwomoh L, Abdelmalik AM, Nilsen M, Stoffels M, Nagel F, Schulz S, Tobin AB, Milligan G. Phosphorylation bar-coding of free fatty acid receptor 2 is generated in a tissue-specific manner. Elife. 2023 Dec 12;12:RP91861. doi: 10.7554/eLife.91861. PMID: 38085667; PMCID: PMC10715726.

Ruhnke N, Beyer AL, Kaemmerer D, Sänger J, Schulz S, Lupp A. Expression of free fatty acid receptor 2 in normal and neoplastic tissues. Exp Mol Pathol. 2024 Jun;137:104902. doi: 10.1016/j.yexmp.2024.104902. Epub 2024 May 23. PMID: 38788249.

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