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pT263/pT264-GPR84 (phospho-GPR84 Antibody)

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Agonist-induced Threonine263/Threonine264 phosphorylation of GPR84
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  • 7TM0120B
  • 100 µl
  • Rabbit
Threonine263/Threonine264 (T263/T264) is major phosphorylation site of human GPR84 receptor. The... more

Threonine263/Threonine264 (T263/T264) is major phosphorylation site of human GPR84 receptor. The pT263/pT264-GPR84 antibody detects phosphorylation in response to agonists. T263/T264 phosphorylation is likely to be involved in efficient ligand sequestration by GPR84.

  Alternative Names GPR84, G Protein-coupled Receptor 84 IUPHAR Target... more

 

Alternative Names GPR84, G Protein-coupled Receptor 84
IUPHAR Target ID  120
UniProt ID  Q9NQS5
Western Blot (WB)  1:1000
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic phosphopeptide derived from human GPR84 around the phosphorylation site of Thr263/Thr264.
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Agonist-induced Threonine263/Threonine264 phosphorylation of GPR84. Upper panel ,... more

Figure 1. Agonist-induced Threonine263/Threonine264 phosphorylation of GPR84. Upper panel, parental or Flp-In TREx 293 harbouring human GPR84-eYFP induced to express the receptor construct (+ dox) were treated with vehicle or 2-HTP for 5 min. Cells were lysed and receptor constuct enriched with a GFP-trap before immunoblotting with the pThr263/pThr264-antibody (7TM0120B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-GFP84 antibody (7TM0120N) at a dilution of 1:1000 to confirm equal loading of the gel.

Marsango S, Ward RJ, Jenkins L, Butcher AJ, Al Mahmud Z, Dwomoh L, Nagel F, Schulz S, Tikhonova... more

Marsango S, Ward RJ, Jenkins L, Butcher AJ, Al Mahmud Z, Dwomoh L, Nagel F, Schulz S, Tikhonova IG, Tobin AB, Milligan G. Selective phosphorylation of threonine residues defines GPR84-arrestin interactions of biased ligands. J Biol Chem. 2022 May;298(5):101932. doi: 10.1016/j.jbc.2022.101932. Epub 2022 Apr 12. PMID: 35427647; PMCID: PMC9118924.

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