![Agonist-induced Serine221/Serine224 phosphorylation of GPR84](https://7tmantibodies.com/media/image/e2/bc/24/pT221-pT224-GPR84-F1_600x600.png)
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- Order number: 7TM0120A
- Content: 100 µl
- Host: Rabbit
Serine221/Serine224 (S221/S224) is major phosphorylation site of human GPR84 receptor. The pS221/pS224-GPR84 antibody detects phosphorylation in response to agonists. S221/S224 phosphorylation is likely to be involved in efficient ligand sequestration by GPR84.
Alternative Names | GPR84, G Protein-coupled Receptor 84 |
IUPHAR Target ID | 120 |
UniProt ID | Q9NQS5 |
Western Blot (WB) | 1:1000 |
Species Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Immunogen | A synthetic phosphopeptide derived from human GPR84 around the phosphorylation site of Ser221/Ser224. |
Form | Liquid |
Purification | Antigen affinity chromatography |
Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Agonist-induced Serine221/Serine224 phosphorylation of GPR84. Upper panel, parental or Flp-In TREx 293 harbouring human GPR84-eYFP induced to express the receptor construct (+ dox) were treated with vehicle or 2-HTP for 5 min. Cells were lysed and receptor constuct enriched with a GFP-trap before immunoblotting with the anti pSer221/pSer224 (7TM0120A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-GFP84 antibody (7TM0120N) at a dilution of 1:1000 to confirm equal loading of the gel.
Marsango S, Ward RJ, Jenkins L, Butcher AJ, Al Mahmud Z, Dwomoh L, Nagel F, Schulz S, Tikhonova IG, Tobin AB, Milligan G. Selective phosphorylation of threonine residues defines GPR84-arrestin interactions of biased ligands. J Biol Chem. 2022 May;298(5):101932. doi: 10.1016/j.jbc.2022.101932. Epub 2022 Apr 12. PMID: 35427647; PMCID: PMC9118924.