pT329/pS331-GAL2 (phospho-Galanin Receptor 2 Antibody)

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Agonist-induced Threonine329/Serine331 phosphorylation of GAL2 Receptor

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Order number: 7TM0244A
Content: 100 µl
Host: Rabbit

Threonine329/Serine331 (T329/S331) is a major phosphorylation site of the GAL2 receptor.... more

Threonine329/Serine331 (T329/S331) is a major phosphorylation site of the GAL2 receptor. The pT329/pS331-GAL2 antibody detects phosphorylation in response to high-efficacy agonists. T329/S331 phosphorylation is a key regulator of GAL2 desensitization, β-arrestin recruitment and internalization.

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Alternative Names GALNR2, Galanin Receptor 2 IUPHAR Target ID... more

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Alternative Names	GALNR2, Galanin Receptor 2
IUPHAR Target ID	244
UniProt ID	O43603
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	-
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human GAL2 around the phosphorylation site Thr329/Ser331.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Threonine329/Serine331 phosphorylation of GAL2 Receptor. Upper... more

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Figure 1. Agonist-induced Threonine329/Serine331 phosphorylation of GAL2 Receptor. Upper panel, HEK293 cells stably expressing the GAL2-Receptor (GAL2) were either not exposed or exposed to 1 µM of specific GAL-Receptor agonist Galanin for 30 minutes. Cells were lysed and immunoblotted with the anti-pT329/pS331-GAL2 antibody (7TM0244A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-GAL2 antibody to confirm equal loading of the gel.

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