pS362/pS363-GHSR (phospho-Ghrelin Receptor Antibody)

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Agonist-induced Serine362/Serine363 phosphorylation of Ghrelin Receptor

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Order number: 7TM0246A
Content: 100 µl
Host: Rabbit

Serine362/Serine363 (S362/S363) is major phosphorylation site of the Ghrelin Receptor (GHSR).... more

Serine362/Serine363 (S362/S363) is major phosphorylation site of the Ghrelin Receptor (GHSR). The pS362/pS363-GHSR antibody detects phosphorylation in response to agonists. S362/S363 phosphorylation is likely to be involved in efficient ligand sequestration by GHSR.

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Alternative Names Growth Hormone-Releasing Peptide Receptor, Ghrelin... more

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Alternative Names	Growth Hormone-Releasing Peptide Receptor, Ghrelin Receptor 1a
IUPHAR Target ID	246
UniProt ID	Q92847
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	-
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human GHSR around the phosphorylation site Ser362/Ser363.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Serine362/Serine363 phosphorylation of Ghrelin Receptor. Upper... more

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Figure 1. Agonist-induced Serine362/Serine363 phosphorylation of Ghrelin Receptor. Upper panel, HEK293 cells stably expressing the Ghrelin Receptor (GHSR) were either not exposed or exposed to 1 µM of specific GHSR agonist Ghrelin for 30 minutes. Cells were lysed and immunoblotted with the anti-pS362/pS363-GHSR antibody (7TM0246A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-GHSR antibody to confirm equal loading of the gel.

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