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- Order number: 7TM0300A
- Content: 100 µl
- Host: Rabbit
Serine361 (S361) is major phosphorylation site of the Neuropeptide FF Receptor 1 (NPFF1). The pS361-NPFF1 antibody detects phosphorylation in response to agonists. S361 phosphorylation is likely to be involved in efficient ligand sequestration by NPFF1.
| Alternative Names | NPFF1R1, RF amide-related Peptide Receptor OT7T022 |
| IUPHAR Target ID | 300 |
| UniProt ID | Q9GZQ6 |
| Western Blot (WB) | 1:1000 |
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Immunogen | A synthetic phosphopeptide derived from human NPFF1 around the phosphorylation site of Ser361. |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
| Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Agonist-induced Serine361 phosphorylation of the Neuropeptide FF Receptor 1. Upper panel, HEK293 cells stably expressing the Neuropeptide FF receptor 1 (NPFF1) were either not exposed or exposed to 100 nM NPFF for 30 minutes. Cells were lysed and immunoblotted with the anti-pS361-NPFF1 antibody (7TM0300A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-NPFF1 antibody to confirm equal loading of the gel.
Be the first to analyze the NPFF1 phosphorylation using our anti-pS361-NPFF1 antibody and let us know.



