pS346-NOP (phospho-Nociceptin/Orphanin FQ Receptor Antibody)

Preview: Agonist-induced Serine346 phosphorylation of the Nociceptin/Orphanin FQ Receptor.

Preview: Analysis of dose-dependent Nociceptin/Orphanin FQ Receptor phosphorylation using a panel of phosphosite-specific antibodies.

Agonist-induced Serine346 phosphorylation of the Nociceptin/Orphanin FQ Receptor.

Analysis of dose-dependent Nociceptin/Orphanin FQ Receptor phosphorylation using a panel of phosphosite-specific antibodies.

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Order number: 7TM0320A
Content: 100 µl
Host: Rabbit

Serine346 (S346) is a major phosphorylation site of the nociceptin/orphanin FQ peptide receptor... more

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Serine346 (S346) is a major phosphorylation site of the nociceptin/orphanin FQ peptide receptor (NFQ receptor, NOP). The pS346-NOP antibody detects phosphorylation in response to agonists as well as after PKC activation. S346 phosphorylation is a key regulator of NOP desensitization, β-arrestin recruitment and internalization.

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Alternative Names NOP, OPRL1, Nociceptin/Orphanin FQ Receptor IUPHAR... more

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Alternative Names	NOP, OPRL1, Nociceptin/Orphanin FQ Receptor
IUPHAR Target ID	320
UniProt ID	P41146 (human) P35377 (mouse) P35370 (rat)
Western Blot (WB)	1:1000
Species Reactivity	Human, Mouse, Rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human NOP around the phosphorylation site of Ser346
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Serine346 phosphorylation of the Nociceptin/Orphanin FQ Receptor.... more

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Figure 1. Agonist-induced Serine346 phosphorylation of the Nociceptin/Orphanin FQ Receptor. Upper panel, HEK293 cells stably expressing the Nociceptin/Orphanin FQ Receptor (NOP) were either not exposed or exposed to 10 μM Nociceptin/Orphanin FQ or 0.1 μM PMA (Phorbol 12-Myristate 13-Acetate) for 30 minutes. Cells were lysed and immunoblotted with the anti-pS346-NOP antibody (7TM0320A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-NOP antibody (7TM0320N-WB) at a dilution of 1:1000 to confirm equal loading of the gel.

Figure 2. Analysis of dose-dependent Nociceptin/Orphanin FQ Receptor phosphorylation using a panel of phosphosite-specific antibodies. Upper three panels, HEK293 cells stably expressing the Nociceptin/Orphanin FQ Receptor (NOP) were either not exposed or exposed to increasing concentrations of Nociceptin/Orphanin FQ ranging from 1 nM to 10 μM for 30 minutes. Cells were lysed and immunoblotted with the anti-pS346-NOP antibody (7TM0320A) or anti-pS351-NOP antibody (7TM0320B) or anti-pT362/pS363-NOP antibody (7TM0320C) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-NOP antibody (7TM0320N-WB) at a dilution of 1:1000 to confirm equal loading of the gel.

Mann A, Moulédous L, Froment C, O'Neill PR, Dasgupta P, Günther T, Brunori G, Kieffer BL, Toll... more

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Mann A, Moulédous L, Froment C, O'Neill PR, Dasgupta P, Günther T, Brunori G, Kieffer BL, Toll L, Bruchas MR, Zaveri NT, Schulz S. Agonist-selective NOP receptor phosphorylation correlates in vitro and in vivo and reveals differential post-activation signaling by chemically diverse agonists. Sci Signal. 2019 Mar 26;12(574). pii: eaau8072. doi: 10.1126/scisignal.aau8072. PubMed PMID: 30914485; PubMed Central PMCID: PMC6934085.

Dasgupta P, Mann A, Polgar WE, Reinscheid RK, Zaveri NT, Schulz S. Attenuated G protein signaling and minimal receptor phosphorylation as a biochemical signature of low side-effect opioid analgesics. Sci Rep. 2022 May 3;12(1):7154. doi: 10.1038/s41598-022-11189-6. PMID: 35504962; PMCID: PMC9065038.

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