NOP (non-phospho), Nociceptin/Orphanin FQ Receptor Antibody

KO-Validated

Preview: Validation of the Nociceptin/Orphanin FQ Receptor in transfected HEK293 cells.

Preview: Analysis of dose-dependent Nociceptin/Orphanin FQ Receptor phosphorylation in vivo in NOP-eGFP knock-in mice using two phosphosite-specific antibodies.

Validation of the Nociceptin/Orphanin FQ Receptor in transfected HEK293 cells.

Analysis of dose-dependent Nociceptin/Orphanin FQ Receptor phosphorylation in vivo in NOP-eGFP knock-in mice using two phosphosite-specific antibodies.

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Order number: 7TM0320N
Content: 100 µl
Host: Rabbit

The non-phospho-NFQ receptor (NOP) antibody is directed against the distal end of the... more

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The non-phospho-NFQ receptor (NOP) antibody is directed against the distal end of the carboxyl-terminal tail of mouse, rat and human NOP. It can be used to detect total NOP receptors in Western blots independent of phosphorylation. The non-phospho-NOP antibody has been validated using knockout mice (KO-Validated).

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Alternative Names NOP, OPRL1, Nociceptin/Orphanin FQ Receptor IUPHAR... more

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Alternative Names	NOP, OPRL1, Nociceptin/Orphanin FQ Receptor
IUPHAR Target ID	320
UniProt ID	P41146 (human) P35377 (mouse) P35370 (rat)
Western Blot (WB)	1:1000
Species Reactivity	Human, Mouse, Rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide presents carboxyl-terminal tail of human NOP
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Validation of the Nociceptin/Orphanin FQ Receptor in transfected HEK293 cells.... more

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Figure 1. Validation of the Nociceptin/Orphanin FQ Receptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Nociceptin/Orphanin FQ Receptor (NOP) were lysed and immunoblotted with the phosphorylation-independent anti-NOP antibody (7TM0320N-WB) at a dilution of 1:1000.

Figure 2. Analysis of dose-dependent Nociceptin/Orphanin FQ Receptor phosphorylation in vivo in NOP-eGFP knock-in mice using two phosphosite-specific antibodies. Upper two panels, NOP-eGFP knock-in mice stably expressing the Nociceptin/Orphanin FQ Receptor tagged with enhanced green fluorescent protein (NOP-eGFP) were intraperitoneal injected with 0.9% NaCl or increasing concentrations of specific Nociceptin/Orphanin FQ receptor agonist AT-202 ranging from 0.3 to 30 mg/kg for 30 minutes. Mice were euthanized and brains were removed. The NOP-eGFP receptor was immunoprecipitated with anti-GFP protein agarose beads and immunoblotted with the anti-pS351-NOP antibody (7TM0320B) or anti-pT362/pS363-NOP antibody (7TM0320C) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-NOP antibody (7TM0320N-WB) at a dilution of 1:1000 to confirm equal loading of the gel.

Mann A, Moulédous L, Froment C, O'Neill PR, Dasgupta P, Günther T, Brunori G, Kieffer BL,... more

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Mann A, Moulédous L, Froment C, O'Neill PR, Dasgupta P, Günther T, Brunori G, Kieffer BL, Toll L, Bruchas MR, Zaveri NT, Schulz S. Agonist-selective NOP receptor phosphorylation correlates in vitro and in vivo and reveals differential post-activation signaling by chemically diverse agonists. Sci Signal. 2019 Mar 26;12(574). pii: eaau8072. doi: 10.1126/scisignal.aau8072. PubMed PMID: 30914485; PubMed Central PMCID: PMC6934085.

Dasgupta P, Mann A, Polgar WE, Reinscheid RK, Zaveri NT, Schulz S. Attenuated G protein signaling and minimal receptor phosphorylation as a biochemical signature of low side-effect opioid analgesics. Sci Rep. 2022 May 3;12(1):7154. doi: 10.1038/s41598-022-11189-6. PMID: 35504962; PMCID: PMC9065038.

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