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pS393/pS394-OX1 (phospho-Orexin Receptor 1 Antibody)

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Agonist-induced Serine393/Serine394 phosphorylation of Orexin Receptor 1
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  • 7TM0321B
  • 100 µl
  • Rabbit
Serine393/Serine394 (S393/S394) is a major phosphorylation site of the Orexin receptor 1 (OX1).... more

Serine393/Serine394 (S393/S394) is a major phosphorylation site of the Orexin receptor 1 (OX1). The pS393/pS394-OX1 antibody detects phosphorylation in response to high-efficacy agonists. S393/S394 phosphorylation is a key regulator of OX1 desensitization, β-arrestin recruitment and internalization.

  Alternative Names OX1R, Hypocretin Receptor 1 IUPHAR Target ID... more

 

Alternative Names OX1R, Hypocretin Receptor 1
IUPHAR Target ID  321
UniProt ID  O43613
Western Blot (WB)  1:1000
Immunocytochemistry (ICC)  -
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic phosphopeptide derived from human OX1 around the phosphorylation site Ser393/Ser394.
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Agonist-induced Serine393/Serine394 phosphorylation of Orexin Receptor 1. Upper... more

Figure 1. Agonist-induced Serine393/Serine394 phosphorylation of Orexin Receptor 1. Upper panel, HEK293 cells stably expressing the Orexin Receptor 1 (OX1) were either not exposed or exposed to 100 nM Orexin-B for 30 minutes. Cells were lysed and immunoblotted with the anti-pS393/pS394-OX1 antibody (7TM0321B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-OX1 antibody to confirm equal loading of the gel.

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