![Agonist-induced Threonine401/Serine403 phosphorylation of Orexin Receptor 2](https://7tmantibodies.com/media/image/18/d5/ec/pT401-pS403-OX2-F1_600x600.png)
Prices plus VAT plus shipping costs
Ready to ship today,
Delivery time appr. 5-8 days
- Order number: 7TM0322A
- Content: 100 µl
- Host: Rabbit
Threonine401/Serine403 (T401/S403) is a major phosphorylation site of the Orexin receptor 2 (OX2). The pT401/pS403-OX2 antibody detects phosphorylation in response to high-efficacy agonists. T401/S403 phosphorylation is a key regulator of OX2 desensitization, β-arrestin recruitment and internalization.
Alternative Names | OX2R, Hypocretin Receptor 2 |
IUPHAR Target ID | 322 |
UniProt ID | O43614 |
Western Blot (WB) | 1:1000 |
Immunocytochemistry (ICC) | - |
Species Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Immunogen | A synthetic phosphopeptide derived from human OX2 around the phosphorylation site Thr401/Ser403. |
Form | Liquid |
Purification | Antigen affinity chromatography |
Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Agonist-induced Threonine401/Serine403 phosphorylation of Orexin Receptor 2. Upper panel, HEK293 cells stably expressing the Orexin Receptor 2 (OX2) were either not exposed or exposed to 100 nM Orexin-B for 30 minutes. Cells were lysed and immunoblotted with the anti-pT401/pS403-OX2 antibody (7TM0322A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-OX2 antibody to confirm equal loading of the gel.