pT342/pT344-EP2 (phospho-EP2 Prostanoid Receptor Antibody)

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Agonist-induced Threonine342/Threonine344 phosphorylation of the EP2 Prostanoid Receptor

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Order number: 7TM0341A
Content: 100 µl
Host: Rabbit

Threonine342/Threonine344 (T342/T344) is major phosphorylation site of the EP2 Prostanoid... more

Threonine342/Threonine344 (T342/T344) is major phosphorylation site of the EP2 Prostanoid Receptor (EP2). The pT342/pT344-EP2 antibody detects phosphorylation in response to agonists. T342/T344 phosphorylation is likely to be involved in efficient ligand sequestration by EP2.

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Alternative Names PGE Receptor EP2 subtype, Prosaglandin E Receptor 2... more

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Alternative Names	PGE Receptor EP2 subtype, Prosaglandin E Receptor 2 (subtype EP2)
IUPHAR Target ID	341
UniProt ID	P43116
Western Blot (WB)	1:1000
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human EP2 around the phosphorylation site of Thr342/Thr344.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Threonine342/Threonine344 phosphorylation of the EP2 Prostanoid... more

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Figure 1. Agonist-induced Threonine342/Threonine344 phosphorylation of the EP2 Prostanoid Receptor. Upper panel, HEK293 cells stably expressing the EP2 Prostanoid receptor (EP2) were either not exposed or exposed to 10 µM Misoprostol for 30 minutes. Cells were lysed and immunoblotted with the anti-pT342/pT344-EP2 antibody (7TM0341A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-EP2 antibody to confirm equal loading of the gel.

Be the first to analyze the EP2 phosphorylation using our anti-pT342/pT344-PAR1... more

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Be the first to analyze the EP2 phosphorylation using our anti-pT342/pT344-PAR1 antibody and let us know.

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