pS369/pS370-EP3 (phospho-EP3 Prostanoid Receptor Antibody)

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Agonist-induced Serine369/Serine370 phosphorylation of the EP3 Prostanoid Receptor

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Order number: 7TM0342A
Content: 100 µl
Host: Rabbit

Serine369/Serine370 (S369/S370) is major phosphorylation site of the EP3 Prostanoid Receptor... more

Serine369/Serine370 (S369/S370) is major phosphorylation site of the EP3 Prostanoid Receptor (EP3). The pS369/pS370-EP3 antibody detects phosphorylation in response to agonists. S369/S370 phosphorylation is likely to be involved in efficient ligand sequestration by EP3.

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Alternative Names PGE Receptor EP3 subtype, Prosaglandin E Receptor 3... more

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Alternative Names	PGE Receptor EP3 subtype, Prosaglandin E Receptor 3
IUPHAR Target ID	342
UniProt ID	P43115
Western Blot (WB)	1:1000
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human EP3 around the phosphorylation site of Ser369/Ser370.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Serine369/Serine370 phosphorylation of the EP3 Prostanoid Receptor.... more

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Figure 1. Agonist-induced Serine369/Serine370 phosphorylation of the EP3 Prostanoid Receptor. Upper panel, HEK293 cells stably expressing the EP3 Prostanoid receptor (EP3) were either not exposed or exposed to 10 µM Misoprostol for 30 minutes. Cells were lysed and immunoblotted with the anti-pS369/pS370-EP3 antibody (7TM0342A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-EP3 antibody to confirm equal loading of the gel.

Be the first to analyze the EP3 phosphorylation using our anti-pS369/pS370-EP3... more

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Be the first to analyze the EP3 phosphorylation using our anti-pS369/pS370-EP3 antibody and let us know.

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