pS324/pS328-IP (phospho-IP Prostacyclin Receptor Antibody)

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Agonist-induced Serine324/Serine328 phosphorylation of the IP Prostacyclin Receptor

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Order number: 7TM0345B
Content: 100 µl
Host: Rabbit

Serine324/Serine328 (S324/S328) is major phosphorylation site of the IP Prostacyclin Receptor... more

Serine324/Serine328 (S324/S328) is major phosphorylation site of the IP Prostacyclin Receptor (IP). The pS324/pS328-IP antibody detects phosphorylation in response to agonists. S324/S328 phosphorylation is likely to be involved in efficient ligand sequestration by IP.

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Alternative Names Prostanoid IP Receptor, Prostaglandin I2 Receptor,... more

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Alternative Names	Prostanoid IP Receptor, Prostaglandin I2 Receptor, Prostacyclin Receptor
IUPHAR Target ID	345
UniProt ID	P43119
Western Blot (WB)	1:1000
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human IP around the phosphorylation site of Ser324/Ser328.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Serine324/Serine328 phosphorylation of the IP Prostacyclin... more

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Figure 1. Agonist-induced Serine324/Serine328 phosphorylation of the IP Prostacyclin Receptor. Upper panel, HEK293 cells stably expressing the IP Prostcyclin receptor (IP) were either not exposed or exposed to 10 µM Treprostinil for 30 minutes. Cells were lysed and immunoblotted with the anti-pS324/pS328-IP antibody (7TM0345B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-IP antibody to confirm equal loading of the gel.

Be the first to analyze the IP phosphorylation using our anti-pS324/pS328-IP... more

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Be the first to analyze the IP phosphorylation using our anti-pS324/pS328-IP antibody and let us know.

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