SST2 (non-phospho), Somatostatin Receptor 2 Antibody

KO-Validated

SST2 (non-phospho), Somatostatin Receptor 2 Antibody

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Order number: 7TM0356N
Content: 100 µl
Host: Rabbit

The non-phospho-SST2 receptor antibody is directed against the distal end of the... more

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The non-phospho-SST2 receptor antibody is directed against the distal end of the carboxyl-terminal tail of mouse, rat and human SST2. It detects selectively the canonical form of SST2 (also referred to as SST2A) and not the putative splice variant SST2B. In can be used to detect total SST2 receptors in Western blots independent of phosphorylation. The non-phospho-SST2 antibody can also be used to isolate and enrich SST2 receptors from tissue lysates. It also detects SST2 in cultured cells and tissue sections by immunohistochemistry. The non-phospho-SST2 antibody has been validated using knockout mice (KO-Validated).

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Alternative Names SST2, SSTR2, Somatostatin Receptor 2 IUPHAR... more

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Alternative Names	SST2, SSTR2, Somatostatin Receptor 2
IUPHAR Target ID	356
Uniprot ID	P30874 (human) P30875 (mouse) P30680 (rat)
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	1:200
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human, Mouse, Rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide corresponding to residues 335–369 of human, mouse and rat SST2
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Validation of the Somatostatin Receptor 2 in transfected HEK293 cells. Native... more

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Figure 1. Validation of the Somatostatin Receptor 2 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Somatostatin Receptor 2 (SST2) were lysed and immunoblotted with the phosphorylation-independent anti-SST2 antibody (7TM0356N) at a dilution of 1:1000.

Figure 2. Immunohistochemical identification of Somatostatin Receptor 2 in human neuroendocrine tumor tissue. Sections were dewaxed, microwaved in citric acid, and incubated with anti-SST2 (non-phospho-Somatostatin Receptor 2) antibody (7TM0356N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, SST2 receptors were uniformly detected at the plasma membrane of nearly all tumor cells.

Schulz S, Pauli SU, Schulz S, Händel M, Dietzmann K, Firsching R, Höllt V. Immunohistochemical... more

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Schulz S, Pauli SU, Schulz S, Händel M, Dietzmann K, Firsching R, Höllt V. Immunohistochemical determination of five somatostatin receptors in meningioma reveals frequent overexpression of somatostatin receptor subtype sst2A. Clin Cancer Res. 2000 May;6(5):1865-74. PubMed PMID: 10815909.

Asnacios A, Courbon F, Rochaix P, Bauvin E, Cances-Lauwers V, Susini C, Schulz S, Boneu A, Guimbaud R, Buscail L. Indium-111-pentetreotide scintigraphy and somatostatin receptor subtype 2 expression: new prognostic factors for malignant well-differentiated endocrine tumors. J Clin Oncol. 2008 Feb 20;26(6):963-70. doi: 10.1200/JCO.2007.12.7431. PubMed PMID: 18281671.

Fischer T, Doll C, Jacobs S, Kolodziej A, Stumm R, Schulz S. Reassessment of sst2 somatostatin receptor expression in human normal and neoplastic tissues using the novel rabbit monoclonal antibody UMB-1. J Clin Endocrinol Metab. 2008 Nov;93(11):4519-24. doi: 10.1210/jc.2008-1063. Epub 2008 Aug 12. PubMed PMID: 18697876.

Lesche S, Lehmann D, Nagel F, Schmid HA, Schulz S. Differential effects of octreotide and pasireotide on somatostatin receptor internalization and trafficking in vitro. J Clin Endocrinol Metab. 2009 Feb;94(2):654-61. doi: 10.1210/jc.2008-1919. Epub 2008 Nov 11. PubMed PMID: 19001514.

Shi TJ, Xiang Q, Zhang MD, Barde S, Kai-Larsen Y, Fried K, Josephson A, Glück L, Deyev SM, Zvyagin AV, Schulz S, Hökfelt T. Somatostatin and its 2A receptor in dorsal root ganglia and dorsal horn of mouse and human: expression, trafficking and possible role in pain. Mol Pain. 2014 Feb 13;10:12. doi: 10.1186/1744-8069-10-12. PubMed PMID: 24521084; PubMed Central PMCID: PMC3943448.

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