SST2 (non-phospho), Somatostatin Receptor 2 Antibody

Figure 1. Western blot analysis of Somatostatin Receptor 2 in mouse brain in vivo. Brains from Somatostatin Receptor 2-deficient mice (SST2-KO) and their wild-type littermates (SST2-WT) were dissected, homogenated and immunoblotted using anti-SST2 (non-phospho-Somatostatin-Receptor 2) antibody (7TM0356N-WB) at a dilution of 1:1000. Note, absence of SST2 receptors in SST-KO but not in SST2-WT mice.

Figure 2. Immunocytochemical identification of Somatostatin Receptor 2 in HEK293 cells. HEK293 cells stably expressing the Somatostatin Receptor 2 (SST2) were either not exposed or exposed to 1 μM SRIF (somatotropin release inhibiting factor) for 30 min and immunocytochemically stained with anti-SST2 (non-phospho-Somatostatin Receptor 2) antibody (7TM0356N-WB) at a dilution of 1:200. Note, SST2 receptors were confined to the plasma membrane in untreated cells (0 min). SST2 receptors were seen in perinuclear clusters of vesicles after 30 min SRIF exposure.

Figure 3. Immunofluorescent identification of  Somatostatin Receptor 2 in human enteric ganglia cells. Sections were dewaxed, microwaved in citric acid, and incubated with a mixtures of anti-SST2 (non-phospho-Somatostatin Receptor 2) antibody (7TM0356N-WB)(Red) at a dilution of 1:100 and a mouse anti-SRIF antibody (Green). Note, SST2 receptors were detected at the plasma membrane of enteric ganglia cells in close proximity to SRIF-containing nerve fibers and terminals.

Figure 4. Immunohistochemical identification of Somatostatin Receptor 2 in human pancreatic islets. Sections were dewaxed, microwaved in citric acid, and incubated with anti-SST2 (non-phospho-Somatostatin Receptor 2) antibody (7TM0356N-WB) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, SST2 receptors were detected at the plasma membrane of nearly all cells in human pancreatic islets.

Figure 5. Immunohistochemical identification of Somatostatin Receptor 2 in human neuroendocrine tumor tissue. Sections were dewaxed, microwaved in citric acid, and incubated with anti-SST2 (non-phospho-Somatostatin Receptor 2) antibody (7TM0356N-WB) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, SST2 receptors were uniformly detected at the plasma membrane of nearly all tumor cells.

Figure 6. Immunohistochemical identification of Somatostatin Receptor 2 in human growth-hormone-producing pituitary adenoma tissue. Sections were dewaxed, microwaved in citric acid, and incubated with anti-SST2 (non-phospho-Somatostatin Receptor 2) antibody (7TM0356N-WB) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, SST2 receptors were uniformly detected at the plasma membrane of nearly all tumor cells.

Figure 7. Immunohistochemical identification of Somatostatin Receptor 2 in human anterior pituitary. Sections were dewaxed, microwaved in citric acid, and incubated with anti-SST2 (non-phospho-Somatostatin Receptor 2) antibody (7TM0356N-WB) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, SST2 receptors were detected on a distinct population of cells in the anterior pituitary.

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