SST3 (non-phospho), Somatostatin Receptor 3 Antibody

Preview: Validation of the Somatostatin Receptor 3 in transfected HEK293 cells.

Preview: Immunocytochemical identification of Somatostatin Receptor 3 in HEK293 cells.

Preview: Immunohistochemical identification of Somatostatin Receptor 3 in human growth-hormone-producing pituitary adenoma tissue.

Validation of the Somatostatin Receptor 3 in transfected HEK293 cells.

Immunocytochemical identification of Somatostatin Receptor 3 in HEK293 cells.

Immunohistochemical identification of Somatostatin Receptor 3 in human growth-hormone-producing pituitary adenoma tissue.

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Order number: 7TM0357N
Content: 100 µl
Host: Rabbit

The non-phospho-SST3 receptor antibody is directed against the distal end of the... more

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The non-phospho-SST3 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human SST3. In can be used to detect total SST3 receptors in Western blots independent of phosphorylation. The non-phospho-SST3 antibody can also be used to isolate and enrich SST3 receptors from tissue lysates. It also detects SST3 in cultured cells and tissue sections by immunohistochemistry.

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Alternative Names SST3, SSTR3, Somatostatin Receptor 3 IUPHAR... more

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Alternative Names	SST3, SSTR3, Somatostatin Receptor 3
IUPHAR Target ID	357
UniProt ID	P32745
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	1:200
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide with sequence QLLPQEASTGEKSSTMRISYL corresponds to amino acids 398-418 in human SST3
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Validation of the Somatostatin Receptor 3 in transfected HEK293 cells. Native... more

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Figure 1. Validation of the Somatostatin Receptor 3 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Somatostatin Receptor 3 (SST3) were lysed and immunoblotted with the phosphorylation-independent anti-SST3 antibody (7TM0357N-WB) at a dilution of 1:1000.

Figure 2. Immunocytochemical identification of Somatostatin Receptor 3 in HEK293 cells. HEK293 cells stably expressing the Somatostatin Receptor 3 (SST3) were either not exposed or exposed to 1 μM SRIF (somatotropin release inhibiting factor) for 30 min and immunocytochemically stained with anti-SST3 (non-phospho-Somatostatin Receptor 3) antibody (7TM0357N-WB) at a dilution of 1:200. Note, SST3 receptors were confined to the plasma membrane in untreated cells (0 min). SST3 receptors were seen in perinuclear clusters of vesicles after 30 min SRIF exposure.

Figure 3. Immunohistochemical identification of Somatostatin Receptor 3 in human growth-hormone-producing pituitary adenoma tissue. Sections were dewaxed, microwaved in citric acid, and incubated with anti-SST3 (non-phospho-Somatostatin Receptor 3) antibody (7TM0357N-WB) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, SST3 receptors were uniformly detected at the plasma membrane of nearly all tumor cells.