pT333-SST5 (phospho-Somatostatin Receptor 5 Antibody)

Preview: Agonist-induced Threonine333 phosphorylation of the Somatostatin Receptor 5.

Preview: Analysis of dose-dependent Somatostatin Receptor 5 phosphorylation.

Agonist-induced Threonine333 phosphorylation of the Somatostatin Receptor 5.

Analysis of dose-dependent Somatostatin Receptor 5 phosphorylation.

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Order number: 7TM0359A
Content: 100 µl
Host: Rabbit

Threonine333 (T333) is a major phosphorylation site of the human somatostatin receptor 5 (SST5).... more

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Threonine333 (T333) is a major phosphorylation site of the human somatostatin receptor 5 (SST5). The pT333-SST5 antibody detects phosphorylation in response to high-efficacy agonists such as pasireotide but not after activation of PKC. T333 phosphorylation is a key regulator of SST5 desensitization, trafficking and signalling.

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Alternative Names SST5, SSTR5, Somatostatin Receptor 5 IUPHAR... more

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Alternative Names	SST5, SSTR5, Somatostatin Receptor 5
IUPHAR Target ID	359
UniProt ID	P35346
Western Blot (WB)	1:1000
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human SST5 around the phosphorylation site of Thr333
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Threonine333 phosphorylation of the Somatostatin Receptor 5. Upper... more

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Figure 1. Agonist-induced Threonine333 phosphorylation of the Somatostatin Receptor 5. Upper panel, HEK293 cells stably expressing the Somatostatin Receptor 5 (SST5) were either not exposed or exposed to 1 μM SRIF (somatotropin-release inhibiting factor) or 0.1 μM PMA (Phorbol 12-Myristate 13-Acetate) for 30 minutes. Cells were lysed and immunoblotted with the anti-pT333-SST5 antibody (7TM0359A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-SST5 antibody (7TM0359N-WB) at a dilution of 1:1000 to confirm equal loading of the gel.

Figure 2. Analysis of dose-dependent Somatostatin Receptor 5 phosphorylation. Upper panel, HEK293 cells stably expressing the Somatostatin Receptor 5 (SST5) were either not exposed or exposed to increasing concentrations of SRIF (somatotropin-release inhibiting factor) ranging from 1 nM to 1 μM for 30 minutes. Cells were lysed and immunoblotted with the anti-pT333-SST5 antibody (7TM0359A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-SST5 antibody (7TM0359N-WB) at a dilution of 1:1000 to confirm equal loading of the gel.

Petrich A, Mann A, Kliewer A, Nagel F, Strigli A, Märtens JC, Pöll F, Schulz S. Phosphorylation... more

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Petrich A, Mann A, Kliewer A, Nagel F, Strigli A, Märtens JC, Pöll F, Schulz S. Phosphorylation of threonine 333 regulates trafficking of the human sst5 somatostatin receptor. Mol Endocrinol. 2013 Apr;27(4):671-82. doi: 10.1210/me.2012-1329. Epub 2013 Feb 15. PubMed PMID: 23418396; PubMed Central PMCID: PMC5416807.

Lehmann A, Kliewer A, Märtens JC, Nagel F, Schulz S. Carboxyl-terminal receptor domains control the differential dephosphorylation of somatostatin receptors by protein phosphatase 1 isoforms. PLoS One. 2014 Mar 17;9(3):e91526. doi: 10.1371/journal.pone.0091526. eCollection 2014. PubMed PMID: 24637622; PubMed Central PMCID: PMC3956607.

Kliewer A, Mann A, Petrich A, Pöll F, Schulz S. A transplantable phosphorylation probe for direct assessment of G protein-coupled receptor activation. PLoS One. 2012;7(6):e39458. doi: 10.1371/journal.pone.0039458. Epub 2012 Jun 26. PubMed PMID: 22745760; PubMed Central PMCID: PMC3383726.

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