pS330/pS331-S1P2 (phospho-Sphingosine 1-Phosphate Receptor 2 Antibody)

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Agonist-induced Serine330/Serine331 phosphorylation of the Sphingosine 1-Phosphate Receptor 2

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Order number: 7TM0276A
Content: 100 µl
Host: Rabbit

Serine330/Serine331 (S330/S331) is major phosphorylation site of the Sphingosine... more

Serine330/Serine331 (S330/S331) is major phosphorylation site of the Sphingosine 1-Phosphate Receptor 2 (S1P2). The pS330/pS331-S1P2 antibody detects phosphorylation in response to agonists. S330/S331 phosphorylation is likely to be involved in efficient ligand sequestration by S1P2.

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Alternative Names S1P Receptor 2, Edg5, endothelial differentiation G... more

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Alternative Names	S1P Receptor 2, Edg5, endothelial differentiation G protein-coupled receptor 5
IUPHAR Target ID	276
UniProt ID	O95136
Western Blot (WB)	1:1000
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human S1P2 around the phosphorylation site of Ser330/Ser331.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Serine330/Serine331 phosphorylation of the Sphingosine 1-Phosphate... more

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Figure 1. Agonist-induced Serine330/Serine331 phosphorylation of the Sphingosine 1-Phosphate Receptor 2. Upper panel, HEK293 cells stably expressing the S1P2-Receptor (S1P2) were either not exposed or exposed to 1 µM of S1P2-Receptor agonists S1P for 30 minutes. Cells were lysed and immunoblotted with the anti-pS330/pS331-S1P2 antibody (7TM00276A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-S1P2 antibody to confirm equal loading of the gel.

Be the first to analyze the S1P1 phosphorylation using our anti-pS351/pS353-S1P1... more

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Be the first to analyze the S1P1 phosphorylation using our anti-pS351/pS353-S1P1 antibody and let us know.

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