pS364/pS366/pS368-S1P4 (phospho-Sphingosine 1-Phosphate Receptor 4 Antibody)

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Agonist-induced Serine364/Serine366/Serine368 phosphorylation of the Sphingosine 1-Phosphate Receptor 4

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Order number: 7TM0278A
Content: 100 µl
Host: Rabbit

Serine364/Serine366/Serine368 (S364/S366/S368) is major phosphorylation site of... more

Serine364/Serine366/Serine368 (S364/S366/S368) is major phosphorylation site of the Sphingosine 1-Phosphate Receptor 4 (S1P4). The pS364/pS366/pS368-S1P4 antibody detects phosphorylation in response to agonists. S364/S366/S368 phosphorylation is likely to be involved in efficient ligand sequestration by S1P4.

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Alternative Names Edg6, endothelial differentiation G protein-coupled... more

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Alternative Names	Edg6, endothelial differentiation G protein-coupled receptor 6
IUPHAR Target ID	278
UniProt ID	O95977
Western Blot (WB)	1:1000
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human S1P4 around the phosphorylation site of Ser364/Ser366/Ser368.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Serine364/Serine366/Serine368 phosphorylation of the Sphingosine... more

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Figure 1. Agonist-induced Serine364/Serine366/Serine368 phosphorylation of the Sphingosine 1-Phosphate Receptor 4. Upper panel, HEK293 cells stably expressing the S1P4-Receptor (S1P4) were either not exposed or exposed to 1 µM of S1P4-Receptor agonists S1P for 30 minutes. Cells were lysed and immunoblotted with the anti-pS364/pS366/pS368-S1P4 antibody (7TM00278A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-S1P4 antibody to confirm equal loading of the gel.

Be the first to analyze the S1P1 phosphorylation using our anti-pS351/pS353-S1P1... more

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Be the first to analyze the S1P1 phosphorylation using our anti-pS351/pS353-S1P1 antibody and let us know.

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