S1P2 (IHC-grade), Sphingosine 1-Phosphate Receptor 2 Antibody

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Preview: Immunohistochemical varification of S1P2 Receptor antibody in human liver.

Preview: Validation of the S1P2 Receptor in transfected HEK293 cells.

Immunohistochemical varification of S1P2 Receptor antibody in human liver.

Validation of the S1P2 Receptor in transfected HEK293 cells.

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Order number: 7TM0276N-IC
Content: 100 µl
Host: Rabbit

The non-phospho-S1P2 receptor antibody is directed against the distal end of the... more

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The non-phospho-S1P2 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human S1P2. It also detects S1P2 in cultured cells and tissue sections by immunohistochemistry. It can be used to detect total S1P2 receptors in Western blots independent of phosphorylation. The S1P2 antibody can also be used to isolate and enrich S1P2 receptors from tissue lysates.

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Alternative Names S1P Receptor 2, Edg5, endothelial differentiation G... more

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Alternative Names	S1P Receptor 2, Edg5, endothelial differentiation G protein-coupled receptor 5
IUPHAR Target ID	276
Uniprot ID	O95136
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide corresponding to c-terminal end of human S1P2.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Immunohistochemical varification of S1P2 Receptor antibody in human liver.... more

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Figure 1. Immunohistochemical varification of S1P2 Receptor antibody in human liver. Sections were dewaxed, microwaved in citric acid, and not exposed (left pannel) or exposed to peptide (right pannel) that was used for production of anti-S1P2 (non-phospho-S1P2 Receptor) antibody (7TM0276N-IC). Sections were then incubated with anti-S1P2 (non-phospho-S1P2 Receptor) antibody (7TM0276N-IC) at a dilution of 1:100 and sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, only in sections without peptide incubation S1P2 receptors were uniformly detected at the plasma membrane of nearly all cells.

Figure 2. Validation of the S1P2 Receptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the S1P2 Receptor (S1P2) were lysed and immunoblotted with the phosphorylation-independent anti-S1P2 antibody (7TM0276N-IC) at a dilution of 1:100.

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