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pT359/pT360-V2 (phospho-Vasopressin Receptor 2 Antibody)

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Agonist-induced Threonine359/Threonine360 phosphorylation of the Vasopressin Receptor 2.
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  • 7TM0368B
  • 100 µl
  • Rabbit
Threonine359/Threonine360 (T359/T360) is major phosphorylation site of the Vasopressin Receptor... more

Threonine359/Threonine360 (T359/T360) is major phosphorylation site of the Vasopressin Receptor 2 (V2). The pT359/pT360-V2 antibody detects phosphorylation in response to agonists. T359/T360 phosphorylation is likely to be involved in efficient ligand sequestration by V2.

  Alternative Names ADHR, DIR3, renal-type arginine vasopressin receptor... more

 

Alternative Names ADHR, DIR3, renal-type arginine vasopressin receptor
IUPHAR Target ID  368
UniProt ID  P30518
Western Blot (WB)  1:1000
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic phosphopeptide derived from human V2 around the phosphorylation site of Thr359/Thr360.
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Agonist-induced Threonine359/Threonine360 phosphorylation of the Vasopressin Receptor... more

Figure 1. Agonist-induced Threonine359/Threonine360 phosphorylation of the Vasopressin Receptor 2. Upper panel, HEK293 cells stably expressing the Vasopressin receptor 2 (V2) were either not exposed or exposed to 100 nM vasopressin for 30 minutes. Cells were lysed and immunoblotted with the anti-pT359/pT360-V2antibody (7TM0368B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-V2 antibody to confirm equal loading of the gel.

 

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