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- Order number: 7TM0366B
- Content: 100 µl
- Host: Rabbit
Threonine386/Serine389 (T368/S389) is major phosphorylation site of the Vasopressin Receptor 1A (V1A). The pT386/pS389-V1A antibody detects phosphorylation in response to agonists. T386/S389 phosphorylation is likely to be involved in efficient ligand sequestration by V1A.
Alternative Names | AVPR1, V1aR, antidiuretic hormone receptor 1a |
IUPHAR Target ID | 366 |
UniProt ID | P37288 |
Western Blot (WB) | 1:1000 |
Species Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Immunogen | A synthetic phosphopeptide derived from human V1A around the phosphorylation site of Thr386/Ser389. |
Form | Liquid |
Purification | Antigen affinity chromatography |
Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Agonist-induced Threonine386/Serine389 phosphorylation of the Vasopressin Receptor 1A. Upper panel, HEK293 cells stably expressing the Vasopressin receptor 1A (V1A) were either not exposed or exposed to 100 nM vasopressin for 30 minutes. Cells were lysed and immunoblotted with the anti-pT386/pS389-V1A antibody (7TM0366B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-V1A antibody to confirm equal loading of the gel.
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