pS261pS262-β2 (phospho-β2-Adrenoceptor Antibody)

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Agonist-induced Serine261/Serine262 phosphorylation of the β2-Adrenoceptor

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Order number: 7TM0029G
Content: 100 µl
Host: Rabbit

Serine261/Serine262 (S261/S262) is a major phosphorylation site of the β2 adrenoceptor.... more

Serine261/Serine262 (S261/S262) is a major phosphorylation site of the β2 adrenoceptor. The pS261/pS262-β2 antibody detects phosphorylation in response to high- and low-efficacy agonists but not after PKC activation. S261/S262 phosphorylation is a key regulator of β2 desensitization, β-arrestin recruitment and internalization.

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Alternative Names β2, B2AR, ADRB2R, β2 Adrenergic... more

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Alternative Names	β2, B2AR, ADRB2R, β2 Adrenergic Receptor, β2-Adrenoceptor
IUPHAR Target ID	29
UniProt ID	P07550
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	-
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human β2 around the phosphorylation site of Ser261/Ser262.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Serine261/Serine262 phosphorylation of the β2-Adrenoceptor.... more

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Figure 1. Agonist-induced Serine261/Serine262 phosphorylation of the β2-Adrenoceptor. Upper panel, HEK293 cells stably expressing the β2-Adrenoceptor (β2) were either not exposed or exposed to 1 µM of specific β2-adrenoceptor agonist Isoprenaline for 30 minutes. Cells were lysed and immunoblotted with the anti-pS261/pS262-β2 antibody (7TM0029G) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-β2 antibody (7TM0029N) at a dilution of 1:1000 to confirm equal loading of the gel.

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