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- Order number: 7TM0040B
- Content: 100 µl
- Host: Rabbit
Threonine363/Threonine360 (T363/T364) is major phosphorylation site of the Bombesin Receptor 3 (BB3). The pT363/pT364-BB3 antibody detects phosphorylation in response to agonists. T363/T364 phosphorylation is likely to be involved in efficient ligand sequestration by BB3.
Alternative Names | BB3, Bombesin like Receptor 3 |
IUPHAR Target ID | 40 |
UniProt ID | P32247 |
Western Blot (WB) | 1:1000 |
Species Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Immunogen | A synthetic phosphopeptide derived from human BB3 around the phosphorylation site of Thr363/Thr364. |
Form | Liquid |
Purification | Antigen affinity chromatography |
Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Agonist-induced Threonine363/Threonine364 phosphorylation of the Bombesin Receptor 3. Upper panel, HEK293 cells stably expressing the Bombesin receptor 3 (BB3) were either not exposed or exposed to 100 nM BB3 agonist BA1 for 30 minutes. Cells were lysed and immunoblotted with the anti-pT363/pT364-BB3 antibody (7TM0040B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-BB3 antibody to confirm equal loading of the gel.
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