Premium Phosphosite-Specific 7TM Antibodies
Novel Tools for Your GPCR Research
Select Your Country of Delivery below

pS320/pS321-CXCR6 (phospho-CXC Chemokine Receptor 6 Antibody)

NEW
$ 375.00 *

Prices plus VAT plus shipping costs

Ready to ship today,
Delivery time appr. 5-8 days

  • 7TM0073A
  • 100 µl
  • Rabbit
Serine320/Serine321 (S320/S321) is a major phosphorylation site of the CXCR6 receptor.... more

Serine320/Serine321 (S320/S321) is a major phosphorylation site of the CXCR6 receptor. The pS320/pS321-CXCR6 antibody detects phosphorylation in response to high- and low-efficacy agonists but not after PKC activation. S320/S321 phosphorylation is a key regulator of CXCR6 desensitization, β-arrestin recruitment and internalization.

  Alternative Names CXCR6, CXC Chemokine Receptor 6, Chemokine (C-X-C motif)... more

 

Alternative Names CXCR6, CXC Chemokine Receptor 6, Chemokine (C-X-C motif) Receptor 6
IUPHAR Target ID  73
UniProt ID  O00574
Western Blot (WB)  1:1000
Immunocytochemistry (ICC)  -
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic phosphopeptide derived from human CXCR6 around the phosphorylation site of Ser320/Ser321
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Agonist-induced Serine320/Serine321 phosphorylation of the CXC Chemokine Receptor 6.... more

Figure 1. Agonist-induced Serine320/Serine321 phosphorylation of the CXC Chemokine Receptor 6. Upper panel, HEK293 cells stably expressing the CXC Chemokine Receptor 6 (CXCR6) were either not exposed or exposed to 100 nM specific CXC Chemokine receptor agonist CXCL16 or 0.1 μM PMA (Phorbol 12-Myristate 13-Acetate) for 30 minutes. Cells were lysed and immunoblotted with the anti-pS320/pS321-CXCR6 antibody (7TM0073A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-CXCR6 antibody to confirm equal loading of the gel.

Figure 2. Analysis of dose-dependent CXC Chemokine Receptor 6 phosphorylation using three phosphosite-specific antibodies. Upper two panels, HEK293 cells stably expressing the CXC Chemokine Receptor 6 (CXCR6) were either not exposed or exposed to increasing concentrations of CXCL16 ranging from 3 nM to 100 nM for 30 minutes. Cells were lysed and immunoblotted with the anti-pS320/pS321-CXCR6 antibody (7TM0073A) or anti-pS325/pT327-CXCR6 antibody (7TM0073B) or anti-pS329/pS331-CXCR6 antibody (7TM0073C) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-CXCR6 antibody to confirm equal loading of the gel.

Be the first to decipher the CXCR6 phosphorylation barcode and let us know. more

Be the first to decipher the CXCR6 phosphorylation barcode and let us know.

Read, write and discuss reviews... more
Customer evaluation for "pS320/pS321-CXCR6 (phospho-CXC Chemokine Receptor 6 Antibody)"
Write an evaluation
Evaluations will be activated after verification.

The fields marked with * are required.

Recently viewed