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pT408/pT409-CCK1 (phospho-Cholecystokinin Receptor 1 Antibody)

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pT408/pT409-CCK1 (phospho-Cholecystokinin Receptor 1 Antibody)
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  • 7TM0076A
  • 100 µl
  • Rabbit
Threonine408/Threonine409 (T408/T409) is a major phosphorylation site of the CCK1 receptor.... more

Threonine408/Threonine409 (T408/T409) is a major phosphorylation site of the CCK1 receptor. The pT408/pT409-CCK1 antibody detects phosphorylation in response to high-efficacy agonists. T408/T409 phosphorylation is a key regulator of CCK1 desensitization, β-arrestin recruitment and internalization.

  Alternative Names CCK1, CCKAR, Cholecystokinin Receptor 1, Cholecystokinin... more

 

Alternative Names CCK1, CCKAR, Cholecystokinin Receptor 1, Cholecystokinin Receptor A
IUPHAR Target ID  76
UniProt ID  P32238
Western Blot (WB)  1:1000
Immunocytochemistry (ICC)  -
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic phosphopeptide derived from human CCK1 around the phosphorylation site Thr408/Thr409.
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Agonist-induced Threonine408/Threonine409 phosphorylation of CCK1 Receptor. Upper... more

Figure 1. Agonist-induced Threonine408/Threonine409 phosphorylation of CCK1 Receptor. Upper panel, HEK293 cells stably expressing the CCK1-Receptor (CCK1) were either not exposed or exposed to 1 µM of specific CCK-Receptor agonist CCK-8 for 30 minutes. Cells were lysed and immunoblotted with the anti-pT408/pT409-CCK1 antibody (7TM0076A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-CCK1 antibody (7TM0076N) at a dilution of 1:1000 to confirm equal loading of the gel.

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