![pT427/pS429-CCK2 (phospho-Cholecystokinin Receptor 2 Antibody)](https://7tmantibodies.com/media/image/62/6e/9c/pT427-pS429-CCK2-F1_600x600.png)
Prices plus VAT plus shipping costs
Ready to ship today,
Delivery time appr. 5-8 days
- Order number: 7TM0077A
- Content: 100 µl
- Host: Rabbit
Threonine427/Serine429 (T427/S429) is a major phosphorylation site of the CCK2 receptor. The pT427/pS429-CCK2 antibody detects phosphorylation in response to high-efficacy agonists. T427/S429 phosphorylation is a key regulator of CRF1 desensitization, β-arrestin recruitment and internalization. T427/S429 also undergoes constitutive phosphorylation.
Alternative Names | CCK2, CCKBR, Cholecystokinin Receptor 2, Cholecystokinin Receptor B |
IUPHAR Target ID | 77 |
UniProt ID | P32239 |
Western Blot (WB) | 1:1000 |
Immunocytochemistry (ICC) | - |
Species Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Immunogen | A synthetic phosphopeptide derived from human CCK2 around the phosphorylation site Thr427/Ser429. |
Form | Liquid |
Purification | Antigen affinity chromatography |
Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Agonist-induced and Agonist-independent Threonine427/Serine429 phosphorylation of CCK2 Receptor. Upper panel, HEK293 cells stably expressing the CCK2-Receptor (CCK2) were either not exposed or exposed to 1 µM of specific CCK-Receptor agonist CCK-8 for 30 minutes. Cells were lysed and immunoblotted with the anti-pT427/pS429-CCK2 antibody (7TM0077A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-CCK2 antibody (7TM0077N) at a dilution of 1:1000 to confirm equal loading of the gel.