D1 [UMB10] (RM-non-phospho), Dopamine Receptor 1 Antibody, Rabbit Monoclonal

Rabbit Monoclonal

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Preview: Validation of the Dopamine Receptor 1 in transfected HEK293 cells

Preview: Immunohistochemical identification of Dopamine Receptor 1 in striatum

Validation of the Dopamine Receptor 1 in transfected HEK293 cells

Immunohistochemical identification of Dopamine Receptor 1 in striatum

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Order number: 7TM0214N-RM
Content: 100 µl
Host: Rabbit

The non-phospho-D1 receptor antibody is directed against the distal part of the... more

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The non-phospho-D1 receptor antibody is directed against the distal part of the carboxyl-terminal tail of human D1. It can be used to detect total D1 receptors in Western blots independent of phosphorylation. The non-phospho-D1 antibody can also be used to isolate and enrich D1 receptors from cell and tissue lysates. It also detects D1 in cultured cells and tissue sections by immunohistochemistry.

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Alternative Names D1, DADR, Dopamine Receptor 1, Dopamine-1A Receptor 1... more

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Alternative Names	D1, DADR, Dopamine Receptor 1, Dopamine-1A Receptor 1
IUPHAR Target ID	214
UniProt ID	P21728
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Monoclonal
Immunogen	A synthetic peptide presents the carboxyl-terminal tail of human D1.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Validation of the Somatostatin Receptor 5 in transfected HEK293 cells. Native... more

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Figure 1. Validation of the Somatostatin Receptor 5 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Somatostatin Receptor 5 (SST5) were lysed and immunoblotted with the phosphorylation-independent anti-SST5 antibody (7TM0359N-RM) at a dilution of 1:1000.

Figure 2. Western blot analysis of Somatostatin Receptor 5 in neuroendocrine tumors. Human tumor samples (T1, T2) from neuroendocrine tumors were homogenated and immunoblotted using anti-SST5 (non-phospho-Somatostatin-Receptor 5) antibody (7TM0359N-RM) at a dilution of 1:1000.

Figure 3. Immunocytochemical identification of Somatostatin Receptor 5 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Somatostatin Receptor (SST5) were immunocytochemically stained with anti-SST5 (non-phospho-Somatostatin Receptor 5) antibody (7TM0359N-RM) at a dilution of 1:200. Note, SST5 receptors were confined to the plasma membrane in tranfected cells.

Figure 4. Immunohistochemical identification of Somatostatin Receptor 5 in human pancreatic islets. Sections were dewaxed, microwaved in citric acid, and incubated with anti-SST5 (non-phospho-Somatostatin Receptor 5) antibody (7TM0359N-RM) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (ACE), and sections were counterstained with hematoxylin. Note, SST5 receptors were detected at the plasma membrane of nearly all cells in human pancreatic islets.

Figure 5. Immunohistochemical identification of Somastatin Receptor 5 in human anterior pituitary. Sections were dewaxed, microwaved in citric acid, and incubated with anti-SST5 (non-phospho-Somatostatin Receptor 5) antibody (7TM0359N-RM) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (ACE), and sections were counterstained with hematoxylin. Note, SST5 receptors were detected on a distinct population of cells in the anterior pituitary.

Figure 6. Immunohistochemical identification of Somastatin Receptor 5 in human growth-hormone-producing pituitary adenoma tissue. Sections were dewaxed, microwaved in citric acid, and incubated with anti-SST5 (non-phospho-Somatostatin Receptor 5) antibody (7TM0359N-ICRM) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation in 3-amino-9-ethylcarbazole (ACE), and sections were counterstained with hematoxylin. Note, SST5 receptors were uniformly detected at the plasma membrane of nearly all tumor cells.

Figure 7. Immunohistochemical identification of Somastatin Receptor 5 in human pheochromocytoma. Sections were dewaxed, microwaved in citric acid, and incubated with anti-SST5 (non-phospho-Somatostatin Receptor 5) antibody (7TM0359N-ICRM) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (ACE), and sections were counterstained with hematoxylin. Note, SST5 receptors were uniformly detected at the plasma membrane of nearly all cells in pheochromocytoma.

Figure 1. Validation of the Dopamine Receptor 1 in transfected HEK293 cells. Native HEK293... more

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Figure 1. Validation of the Dopamine Receptor 1 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Dopamine Receptor 1 (D1) were lysed and immunoblotted with the phosphorylation-independent anti-D1 antibody (7TM0214N-RM) at a dilution of 1:1000.

Figure 2. Immunohistochemical identification of Dopamine Receptor 1 in striatum. Sections were dewaxed, microwaved in citric acid, and incubated with anti-D1 (Dopamine Receptor 1) antibody (7TM0214N-RM) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.