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pS317/pT318-D2 (phospho-Dopamine Receptor 2 Antibody)

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  • 7TM0215A
  • 100 µl
  • Rabbit
Serine317/threonine318 (S317/T318) is a major phosphorylation site of the D2 dopamine receptor.... more

Serine317/threonine318 (S317/T318) is a major phosphorylation site of the D2 dopamine receptor. The pS317/pT318-D2 antibody detects phosphorylation in response to high- and low-efficacy agonists but not after PKC activation. S317/T318 phosphorylation is a key regulator of D2 desensitization, β-arrestin recruitment and internalization.

  Alternative Names D2, D2R, Dopamine Receptor 2 IUPHAR Target ID... more

 

Alternative Names D2, D2R, Dopamine Receptor 2
IUPHAR Target ID  215
UniProt ID  P14416
Western Blot (WB)  1:1000
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic phosphopeptide derived from human D2 around the phosphorylation site of Ser317/Thr318
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Agonist-induced Serine317/Threonine318 phosphorylation of the Dopamine Receptor 2.... more

Figure 1. Agonist-induced Serine317/Threonine318 phosphorylation of the Dopamine Receptor 2. Upper panel, HEK293 cells stably expressing the Dopamine Receptor 2 (D2) were either not exposed or exposed to 10 μM Quinprirole or 0.1 μM PMA (Phorbol 12-Myristate 13-Acetate) for 30 minutes. Cells were lysed and immunoblotted with the anti-pS317/pT318-D2 antibody (7TM0215A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-D2 antibody (7TM0215N-WB) at a dilution of 1:1000 to confirm equal loading of the gel.

Figure 2. Analysis of dose-dependent Dopamine Receptor 2 phosphorylation. Upper panel, HEK293 cells stably expressing the Dopamine Receptor 2 (D2) were either not exposed or exposed to increasing concentrations of Quinpirole ranging from 1 nM to 10 μM for 30 minutes. Cells were lysed and immunoblotted with the anti-pS317/pT318-D2 antibody (7TM0215A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-D2 antibody (7TM0215N-WB) at a dilution of 1:1000 to confirm equal loading of the gel.

Mann A, Keen AC, Mark H, Dasgupta P, Javitch JA, Canals M, Schulz S, Robert Lane J. New... more

Mann A, Keen AC, Mark H, Dasgupta P, Javitch JA, Canals M, Schulz S, Robert Lane J. New phosphosite-specific antibodies to unravel the role of GRK phosphorylation in dopamine D2 receptor regulation and signaling. Sci Rep. 2021 Apr 15;11(1):8288. doi: 10.1038/s41598-021-87417-2. PMID: 33859231; PMCID: PMC8050214.

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