pT328/pS329 FFA3 (phospho-FFA3 Antibody)

NEW

Agonist-induced Threonine328/Serine329 phosphorylation of the FFA Receptor 3

$ 375.00 *

Ready to ship today,
Delivery time appr. 5-8 days

Order number: 7TM0227B
Content: 100 µl
Host: Rabbit

Threonine328/Serine329 (T328/S329) is major phosphorylation site of the FFA Receptor 3 (FFA3).... more

Threonine328/Serine329 (T328/S329) is major phosphorylation site of the FFA Receptor 3 (FFA3). The pT328/pS329-FFA3 antibody detects phosphorylation in response to agonists. T328/S329 phosphorylation is likely to be involved in efficient ligand sequestration by FFA3.

Available downloads:

Alternative Names FFAR3, Free Fatty Acid Receptor 3, GPCR41 IUPHAR... more

Close menu

Alternative Names	FFAR3, Free Fatty Acid Receptor 3, GPCR41
IUPHAR Target ID	227
UniProt ID	O14843
Western Blot (WB)	1:500
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human FFA3 around the phosphorylation site of Thr328/Ser329.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Threonine328/Serine329 phosphorylation of the FFA Receptor 3. Upper... more

Close menu

Figure 1. Agonist-induced Threonine328/Serine329 phosphorylation of the FFA Receptor 3. Upper panel, Flp-In TREx 293 harbouring human FFA2-eYFP induced to express the receptor construct (+ dox) were treated with vehicle, 2mM propanoic acid (C3) or 5µM TUG-1907 for 5 min. Cells were lysed and receptor construct enriched with a GFP-trap, before immunoblotting with the anti-pT328/pS329-FFA3 antibody (7TM0227B) at a dilution of 1:500. Lower panel, blot was stripped and reprobed with a phosphorylation-independent anti-FFA3 antibody to confirm equal loading of the gel. TUG-1097 is described in J. Med Chem 63(7):3577-3595

Close menu

Read, write and discuss reviews... more

Close menu

Customer evaluation for "pT328/pS329-FFA3 (phospho-FFA3 Antibody)"

Write an evaluation

Evaluations will be activated after verification.