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- Order number: 7TM0127N
- Content: 100 µl
- Host: Rabbit
The non-phospho-FFA4 receptor antibody is directed against the distal part of the carboxyl-terminal tail of human FFA4. It can be used to detect total FFA4 receptors in Western blots independent of phosphorylation. The non-phospho-FFA4 antibody can also be used to detect FFA4 in formalin-fixed, paraffin-embedded tissue sections by immunohistochemistry.
Alternative Names | PGPR4, Omega-3 fatty acid Receptor 1, O3FAR1 |
IUPHAR Target ID | 127 |
UniProt ID | Q5NUL3 |
Western Blot (WB) | 1:1000 |
Immunohistochemistry (IHC) | 1:100 |
Species Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Immunogen | A synthetic peptide presents the carboxyl-terminal tail of human FFA4. |
Form | Liquid |
Purification | Antigen affinity chromatography |
Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Detection of non-phosphorylated human FFA4 Receptor. Flp-In TREx 293 harbouring human FFA4-eYFP induced to express the receptor construct were treated with vehicle or 10 µM TUG-891 for 5 min. Cells were lysed and receptor construct enriched with a GFP-trap, before immunoblotting with anti-non-phospho-FFA4 at a dilution of 1:1000 (upper panel) or anti-GFP 1:10,000 to confirm expression and equal loading of the gel (lower panel).
Figure 2. Immunohistochemical identification of FFA4 Receptor in human spleen. Sections were dewaxed, microwaved in citric acid, and incubated with anti-FFA4 antibody (7TM0127N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, FFA4 receptors were detected at the plasma membrane of cells in human spleen.