FFA4 (non-phospho-FFA4 Antibody)

Figure 1. Detection of non-phosphorylated human FFA4 Receptor. Flp-In TREx 293 harbouring human FFA4-eYFP induced to express the receptor construct were treated with vehicle or 10 µM TUG-891 for 5 min. Cells were lysed and receptor construct enriched with a GFP-trap, before immunoblotting with anti-non-phospho-FFA4 at a dilution of 1:1000 (upper panel) or anti-GFP 1:10,000 to confirm expression and equal loading of the gel (lower panel).

Figure 2. Immunohistochemical identification of FFA4 Receptor in human spleen. Sections were dewaxed, microwaved in citric acid, and incubated with anti-FFA4 antibody (7TM0127N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, FFA4 receptors were detected at the plasma membrane of cells in human spleen.