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pT340-GAL1 (phospho-Galanin Receptor 1 Antibody)

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pT340-GAL1 (phospho-Galanin Receptor 1 Antibody)
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  • 7TM0243A
  • 100 µl
  • Rabbit
Threonine340 (T340) is a major phosphorylation site of the GAL1 receptor. The pT340-GAL1... more

Threonine340 (T340) is a major phosphorylation site of the GAL1 receptor. The pT340-GAL1 antibody detects phosphorylation in response to high-efficacy agonists. T340 phosphorylation is a key regulator of GAL1 desensitization, β-arrestin recruitment and internalization.

  Alternative Names GALNR1, GALNR, Galanin Receptor 1 IUPHAR Target ID... more

 

Alternative Names GALNR1, GALNR, Galanin Receptor 1
IUPHAR Target ID  243
UniProt ID  P47211
Western Blot (WB)  1:1000
Immunocytochemistry (ICC)  -
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic phosphopeptide derived from human GAL1 around the phosphorylation site Thr340.
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Agonist-induced Threonine340 phosphorylation of GAL1 Receptor. Upper panel, HEK293... more

Figure 1. Agonist-induced Threonine340 phosphorylation of GAL1 Receptor. Upper panel, HEK293 cells stably expressing the GAL1-Receptor (GAL1) were either not exposed or exposed to 1 µM of specific GAL-Receptor agonist Galanin for 30 minutes. Cells were lysed and immunoblotted with the anti-pT340-GAL1 antibody (7TM0243A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-GAL1 antibody (7TM0243N) at a dilution of 1:1000 to confirm equal loading of the gel.

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