M1 (non-phospho-M1 Muscarinic Acetylcholine Receptor Antibody)

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Validation of the M1 Muscarinic Acetylcholine Receptor in transfected HEK293 cells

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Order number: 7TM0013N
Content: 100 µl
Host: Rabbit

The non-phospho-M1 receptor antibody is directed against the 3rd loop of human M1 Muscarinic... more

The non-phospho-M1 receptor antibody is directed against the 3rd loop of human M1 Muscarinic Acetylcholine Receptor (M1). It can be used to detect total M1 receptors in Western blots independent of phosphorylation. The non-phospho-M1 antibody can also be used to isolate and enrich M1 receptors from cell and tissue lysates.

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Alternative Names M1R, Cholinergic Receptor, Cholinergic Receptor... more

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Alternative Names	M1R, Cholinergic Receptor, Cholinergic Receptor muscarinic 1
IUPHAR Target ID	13
UniProt ID	P11229
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	-
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide presents the third intracellular loop of human M1.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Validation of the M1 Muscarinic Acetylcholine Receptor in transfected HEK293 cells.... more

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Figure 1. Validation of the M1 Muscarinic Acetylcholine Receptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the M1 Muscarinic Acetylcholine Receptor (M1) were lysed and immunoblotted with the phosphorylation-independent anti-M1 antibody (7TM0013N) at a dilution of 1:1000.

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