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- Order number: 7TM0015N
- Content: 100 µl
- Host: Rabbit
The non-phospho-M3 receptor antibody is directed against the distal part of the carboxyl-terminal tail of human M3 Muscarinic Acetylcholine Receptor (M3). It can be used to detect total M3 receptors in Western blots independent of phosphorylation. The non-phospho-M3 antibody can also be used to isolate and enrich M3 receptors from cell and tissue lysates.
Alternative Names | HM4, Chrm 3, Cholinergic Receptor muscarinic 3 |
IUPHAR Target ID | 15 |
UniProt ID | P20309 |
Western Blot (WB) | 1:1000 |
Immunohistochemistry (IHC) | - |
Species Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Immunogen | A synthetic peptide presents the third intracellular loop of human M3. |
Form | Liquid |
Purification | Antigen affinity chromatography |
Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Validation of the M3 Muscarinic Acetylcholine Receptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Muscarinic Acetylcholine Receptor (M3) were lysed and immunoblotted with the phosphorylation-independent anti-M3 antibody (7TM0015N) at a dilution of 1:1000.