pT363-KOP (phospho-κ-Opioid Receptor Antibody)

Preview: Agonist-induced Threonine363 phosphorylation of the κ-Opioid Receptor.

Preview: Analysis of dose-dependent κ-Opioid Receptor phosphorylation using two phosphosite-specific antibodies.

Agonist-induced Threonine363 phosphorylation of the κ-Opioid Receptor.

Analysis of dose-dependent κ-Opioid Receptor phosphorylation using two phosphosite-specific antibodies.

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Order number: 7TM0318B
Content: 100 µl
Host: Rabbit

Threonine363 is a major phosphorylation site of the kappa-opioid receptor (KOP). The pT363-KOP... more

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Threonine363 is a major phosphorylation site of the kappa-opioid receptor (KOP). The pT363-KOP antibody detects phosphorylation in response to high-efficacy agonists. T363 phosphorylation is a key regulator of KOP desensitization, β-arrestin recruitment and internalization.

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Alternative Names KOP, OPRK1, κ-Opioid Receptor IUPHAR Target ID... more

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Alternative Names	KOP, OPRK1, κ-Opioid Receptor
IUPHAR Target ID	318
UniProt ID	P41145 (human) P33534 (mouse) P34975 (rat)
Western Blot (WB)	1:1000
Species Reactivity	Human, Mouse, Rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human KOP around the phosphorylation site of Thr363
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Threonine363 phosphorylation of the κ-Opioid Receptor. Upper panel,... more

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Figure 1. Agonist-induced Threonine363 phosphorylation of the κ-Opioid Receptor. Upper panel, HEK293 cells stably expressing the κ-Opioid Receptor (KOP) were either not exposed or exposed to 10 μM of selective κ-opioid receptor agonist U50,488H for 30 minutes. Cells were lysed and immunoblotted with the anti-pT363-KOP antibody (7TM0318B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-KOP antibody (7TM0318N-WB) to confirm equal loading of the gel.

Figure 2. Analysis of dose-dependent κ-Opioid Receptor phosphorylation using two phosphosite-specific antibodies. Upper two panels, HEK293 cells stably expressing the κ-Opioid Receptor (KOP) were either not exposed or exposed to increasing concentrations of selective κ-opioid receptor agonist U50,488H ranging from 0.1 nM to 1 μM for 30 minutes. Cells were lysed and immunoblotted with the anti-pS356/pT357-KOP antibody (7TM0318A) or anti-pT363-KOP antibody (7TM0318B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-KOP antibody (7TM0318N-WB) to confirm equal loading of the gel.

Chen C, Chiu YT, Wu W, Huang P, Mann A, Schulz S, Liu-Chen LY. Determination of sites of... more

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Chen C, Chiu YT, Wu W, Huang P, Mann A, Schulz S, Liu-Chen LY. Determination of sites of U50,488H-promoted phosphorylation of the mouse κ opioid receptor (KOPR): disconnect between KOPR phosphorylation and internalization. Biochem J. 2016 Feb 15;473(4):497-508. doi: 10.1042/BJ20141471. Epub 2015 Dec 3. PubMed PMID: 26635353; PubMed Central PMCID: PMC5444940.

Chiu YT, Chen C, Yu D, Schulz S, Liu-Chen LY. Agonist-Dependent and -Independent κ Opioid Receptor Phosphorylation: Distinct Phosphorylation Patterns and Different Cellular Outcomes. Mol Pharmacol. 2017 Nov;92(5):588-600. doi: 10.1124/mol.117.108555. Epub 2017 Sep 11. Erratum in: Mol Pharmacol. 2018 Aug;94(2):895. PubMed PMID: 28893975; PubMed Central PMCID: PMC5635518.

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