![Agonist-induced Serine501/Threonine503/Serine504 phosphorylation of the Parathyroid Hormone Receptor 1](https://7tmantibodies.com/media/image/d2/6c/69/pS501-pT503-pT504-PTH1-F1-NEW_600x600.png)
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- Order number: 7TM0331D
- Content: 100 µl
- Host: Rabbit
Serine501/Threonine503/Serine504 (S501/T503/S504) is major phosphorylation site of the Parathyroid Hormone Receptor 1 (PTH1). The pS501pT503/pS504-PTH1 antibody detects phosphorylation in response to agonists. S501T503/S504 phosphorylation is likely to be involved in efficient ligand sequestration by PTH1.
Alternative Names | PPR, PTHR |
IUPHAR Target ID | 331 |
UniProt ID | Q03431 |
Western Blot (WB) | 1:1000 |
Species Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Immunogen | A synthetic phosphopeptide derived from human PTH1 around the phosphorylation site of Ser501/Thr503/Ser504. |
Form | Liquid |
Purification | Antigen affinity chromatography |
Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Agonist-induced Serine501/Threonine503/Serine504 phosphorylation of the Parathyroid Hormone Receptor 1. Upper panel, HEK293 cells stably expressing the Parathyroid Hormone receptor 1 (PTH1) were either not exposed or exposed to 100 nM PTH for 30 minutes. Cells were lysed and immunoblotted with the anti-pS501/pT503/pS504-PTH1 antibody (7TM0331D) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-PTH1 antibody (7TM0331N) to confirm equal loading of the gel.
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