![Agonist-induced Threonine342/Threonine344 phosphorylation of the EP2 Prostanoid Receptor](https://7tmantibodies.com/media/image/71/e2/29/pT342-pT344-EP2-F1AH0wIFTx4gnjb_600x600.png)
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- Order number: 7TM0341A
- Content: 100 µl
- Host: Rabbit
Threonine342/Threonine344 (T342/T344) is major phosphorylation site of the EP2 Prostanoid Receptor (EP2). The pT342/pT344-EP2 antibody detects phosphorylation in response to agonists. T342/T344 phosphorylation is likely to be involved in efficient ligand sequestration by EP2.
Alternative Names | PGE Receptor EP2 subtype, Prosaglandin E Receptor 2 (subtype EP2) |
IUPHAR Target ID | 341 |
UniProt ID | P43116 |
Western Blot (WB) | 1:1000 |
Species Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Immunogen | A synthetic phosphopeptide derived from human EP2 around the phosphorylation site of Thr342/Thr344. |
Form | Liquid |
Purification | Antigen affinity chromatography |
Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Agonist-induced Threonine342/Threonine344 phosphorylation of the EP2 Prostanoid Receptor. Upper panel, HEK293 cells stably expressing the EP2 Prostanoid receptor (EP2) were either not exposed or exposed to 10 µM Misoprostol for 30 minutes. Cells were lysed and immunoblotted with the anti-pT342/pT344-EP2 antibody (7TM0341A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-EP2 antibody to confirm equal loading of the gel.
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