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pS324/pS328-IP (phospho-IP Prostacyclin Receptor Antibody)

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Agonist-induced Serine324/Serine328 phosphorylation of the IP Prostacyclin Receptor
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  • 7TM0345B
  • 100 µl
  • Rabbit
Serine324/Serine328 (S324/S328) is major phosphorylation site of the IP Prostacyclin Receptor... more

Serine324/Serine328 (S324/S328) is major phosphorylation site of the IP Prostacyclin Receptor (IP). The pS324/pS328-IP antibody detects phosphorylation in response to agonists. S324/S328 phosphorylation is likely to be involved in efficient ligand sequestration by IP.

  Alternative Names Prostanoid IP Receptor, Prostaglandin I2 Receptor,... more

 

Alternative Names Prostanoid IP Receptor, Prostaglandin I2 Receptor, Prostacyclin Receptor
IUPHAR Target ID  345
UniProt ID  P43119
Western Blot (WB)  1:1000
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic phosphopeptide derived from human IP around the phosphorylation site of Ser324/Ser328.
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Agonist-induced Serine324/Serine328 phosphorylation of the IP Prostacyclin... more

Figure 1. Agonist-induced Serine324/Serine328 phosphorylation of the IP Prostacyclin Receptor. Upper panel, HEK293 cells stably expressing the IP Prostcyclin receptor (IP) were either not exposed or exposed to 10 µM Treprostinil for 30 minutes. Cells were lysed and immunoblotted with the anti-pS324/pS328-IP antibody (7TM0345B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-IP antibody to confirm equal loading of the gel.

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