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PAR1 (non-phospho), Proteinase-Activated Receptor 1 Antibody

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Validation of the Proteinase-Activated Receptor 1 in transfected HEK293 cells
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  • 7TM0347N
  • 100 µl
  • Rabbit
The PAR1 receptor antibody is directed against the distal end of the carboxyl-terminal tail of... more

The PAR1 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human Proteinase-Activated Receptor 1. It can be used to detect total PAR1 receptors in Western blots independent of phosphorylation. The PAR1 antibody can also be used to isolate and enrich PAR1 receptors from cell and tissue lysates.

  Alternative Names Coagulation Factor II Receptor, Protease-Activated... more

 

Alternative Names Coagulation Factor II Receptor, Protease-Activated Receptor 1
IUPHAR Target ID  347
UniProt ID  P25116
Western Blot (WB)  1:1000
Immunocytochemistry (ICC) 1:200
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic peptide presents part of carboxyl-terminal tail of human PAR1.
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Validation of the Proteinase-Activated Receptor 1 in transfected HEK293 cells.... more

Figure 1. Validation of the Proteinase-Activated Receptor 1 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Proteinase-Activated Receptor 1 (PAR1) were lysed and immunoblotted with the anti-PAR1 antibody (7TM0347N) at a dilution of 1:1000. Note, PAR1 forms stable multimers.

 

 

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