NK1 (IHC-grade), Tachykinin Receptor 1 Antibody

Preview: Validation of the Tachykinin Receptor 1 in transfected HEK293 cells

Preview: Immunocytochemical identification of Tachykinin Receptor 1 in HEK293 cells

Preview: Immunohistochemical identification of Tachykinin Receptor 1 in pancreatic carcinoma

Preview: Immunohistochemical identification of Tachykinin Receptor 1 in ovarian carcinom

Validation of the Tachykinin Receptor 1 in transfected HEK293 cells

Immunocytochemical identification of Tachykinin Receptor 1 in HEK293 cells

Immunohistochemical identification of Tachykinin Receptor 1 in pancreatic carcinoma

Immunohistochemical identification of Tachykinin Receptor 1 in ovarian carcinom

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Order number: 7TM0360N
Content: 100 µl
Host: Rabbit

The NK1 antibody is directed against the distal end of the carboxyl-terminal tail of human... more

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The NK1 antibody is directed against the distal end of the carboxyl-terminal tail of human Tachykinin Receptor 1 (also called Substance P Receptor). It detects selectively the canonical full-length NK1 Tachykinin Receptor. It can be used to detect total NK1 receptors in Western blots independent of phosphorylation. The NK1 antibody can also be used to isolate and enrich NK1 receptors from brain lysates. It also detects NK1 in cultured cells and tissue sections by immunohistochemistry.

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Alternative Names NK1, SPR, TACR1, Neurokinin Receptor 1, Substance P... more

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Alternative Names	NK1, SPR, TACR1, Neurokinin Receptor 1, Substance P Receptor
IUPHAR Target ID	360
UniProt ID	P25103
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	1:200
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic with sequence SSRSDSKTMTESFSFSSNVLS corresponds to amino acids 387-407 of human NK1.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Validation of the Tachykinin Receptor 1 in transfected HEK293 cells. Native HEK293... more

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Figure 1. Validation of the Tachykinin Receptor 1 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Tachykinin Receptor 1 (NK1) were lysed and immunoblotted with the anti-NK1 antibody (7TM0360N) at a dilution of 1:1000.

Figure 2. Immunocytochemical identification of Tachykinin Receptor 1 in HEK293 cells. HEK293 cells stably expressing the Tachykinin receptor 1 (NK1) were either not exposed or exposed to 1 μM Substance P for 30 min and immunocytochemically stained with anti-NK1 (non-phospho-Tachykinin receptor 1) antibody (7TM0360N) at a dilution of 1:200. Note, NK1 receptors were confined to the plasma membrane in untreated cells (0 min). NK1 receptors were seen in perinuclear clusters of vesicles after 30 min Substance P exposure.

Figure 3. Immunohistochemical identification of Tachykinin Receptor 1 in pancreatic carcinoma. Sections were dewaxed, microwaved in citric acid, and incubated with anti-NK1 (Tachykinin Receptor 1) antibody (7TM0360N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, NK1 receptors were uniformly detected at the plasma membrane of chief cell in the pancreatic carcinoma.

Figure 4. Immunohistochemical identification of Tachykinin Receptor 1 in ovarian carcinoma. Sections were dewaxed, microwaved in citric acid, and incubated with anti-NK1 (Tachykinin receptor 1) antibody (7TM0360N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, NK1 receptors were detected predominantly at the level of plasma membrane in ovarian carcinoma.

Schulz S, Stumm R, Röcken C, Mawrin C, Schulz S. Immunolocalization of full-length NK1... more

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Schulz S, Stumm R, Röcken C, Mawrin C, Schulz S. Immunolocalization of full-length NK1 tachykinin receptors in human tumors. J Histochem Cytochem. 2006 Sep;54(9):1015-20. Epub 2006 May 1. PubMed PMID: 16651388.

Blum NK, Schaffner A, Drube J, Nagel F, Reinscheid RK, Hoffmann C, Schulz S. Rapid elucidation of agonist-driven regulation of the neurokinin 1 receptor using a GPCR phosphorylation immunoassay. Eur J Pharmacol. 2024 Jun 15;973:176587. doi: 10.1016/j.ejphar.2024.176587. Epub 2024 Apr 19. PMID: 38642667.

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