V1A (IHC-grade), Vasopressin Receptor 1A Antibody

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Preview: Immunohistochemical identification of Vasopressin Receptor 1A in pancreas

Preview: Validation of the Vasopressin Receptor 1A in transfected HEK293 cells

Immunohistochemical identification of Vasopressin Receptor 1A in pancreas

Validation of the Vasopressin Receptor 1A in transfected HEK293 cells

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Order number: 7TM0366N-IC
Content: 100 µl
Host: Rabbit

The non-phospho-V1A Receptor Antibody is directed against the distal end of the... more

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The non-phospho-V1A Receptor Antibody is directed against the distal end of the carboxyl-terminal tail of human V1A. It can be used to detect total V1A receptors in Western blots independent of phosphorylation. The non-phospho-V1A antibody can also be used to isolate and enrich V1A receptors from cell and tissue lysates. It also detects V1A in cultured cells and tissue sections by immunohistochemistry.

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Alternative Names AVPR1, V1aR, antidiuretic hormone receptor 1a... more

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Alternative Names	AVPR1, V1aR, antidiuretic hormone receptor 1a
IUPHAR Target ID	366
UniProt ID	P37288
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide presents carboxyl-terminal tail of human V1A.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Immunohistochemical identification of Vasopressin Receptor 1A in... more

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Figure 1. Immunohistochemical identification of Vasopressin Receptor 1A in pancreas. Sections were dewaxed, microwaved in citric acid, and incubated with anti-V1A (Vasopressin Receptor 1A) antibody (7TM0366N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin.

Figure 2. Validation of the Vasopressin Receptor 1A in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the vasopressin receptor 1A (V1A) were lysed and immunoblotted with the phosphorylation-independent anti-V1A antibody (7TM0366N-IC) at a dilution of 1:1000.