C5a1 (GP-non-phospho), Complement C5a Receptor 1 Antibody; Guinea Pig

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Preview: Validation of the Complement C5a Receptor 1 in transfected HEK293 cells

Preview: Immunohistochemical identification of Complement C5a Receptor 1 in Pancreas

Preview: Immunohistochemical identification of Complement C5a Receptor 1 in Spleen.

Validation of the Complement C5a Receptor 1 in transfected HEK293 cells

Immunohistochemical identification of Complement C5a Receptor 1 in Pancreas

Immunohistochemical identification of Complement C5a Receptor 1 in Spleen.

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Order number: 7TM0032N-GP
Content: 100 µl
Host: Guinea Pig

The non-phospho-C5a1 receptor antibody is directed against the distal end of the... more

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The non-phospho-C5a1 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human C5a1. It can be used to detect total C5a1 receptors in Western blots independent of phosphorylation. The non-phospho-C5a1 antibody can also be used to isolate and enrich C5a1 receptors from cell and tissue lysates. It also detects C5a1 in cultured cells and tissue sections by immunohistochemistry.

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Alternative Names C5a1, C5AR1, Complement C5a Receptor 1, C5a Anaphylatoxin... more

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Alternative Names	C5a1, C5AR1, Complement C5a Receptor 1, C5a Anaphylatoxin Chemotactic Receptor 1
IUPHAR Target ID	32
UniProt ID	P21730
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human
Host / Isotype	Guinea Pig / IgG
Class	Polyclonal
Immunogen	A synthetic peptide presents carboxyl-terminal tail of human C5a1.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Validation of the Complement C5a Receptor 1 in transfected HEK293 cells. Native... more

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Figure 1. Validation of the Complement C5a Receptor 1 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Complement C5a Receptor 1 (C5a1) were lysed and immunoblotted with the anti-C5a1 antibody (7TM0032N-GP) at a dilution of 1:1000.

Figure 2. Immunohistochemical identification of Complement C5a Receptor 1 in Pancreas. Sections were dewaxed, microwaved in citric acid, and incubated with anti-C5a1 (Complement C5a Receptor 1) antibody (7TM0032N-GP) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-guinea pig IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.

Figure 3. Immunohistochemical identification of Complement C5a Receptor 1 in Spleen. Sections were dewaxed, microwaved in citric acid, and incubated with anti-C5a1 (Complement C5a Receptor 1) antibody (7TM0032N-GP) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-guinea pig IgG and avidin-biotin solution. Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.