NTS1 (GP-IHC-grade), Neurotensin Receptor 1 Antibody, Guinea Pig

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Preview: Immunohistochemical identification of Neurotensin Receptor 1 in Accumbens

Preview: Validation of the Neurotensin Receptor 1 in transfected HEK293 cells

Immunohistochemical identification of Neurotensin Receptor 1 in Accumbens

Validation of the Neurotensin Receptor 1 in transfected HEK293 cells

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Order number: 7TM0309N-ICGP
Content: 100 µl
Host: Guinea Pig

The NTS1 receptor antibody is directed against the distal end of the carboxyl-terminal tail of... more

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The NTS1 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human Neurotensin Receptor 1. It can be used to detect total NTS1 receptors in Western blots independent of phosphorylation. The NTS1 antibody can also be used to isolate and enrich NTS1 receptors from cell and tissue lysates. It also detects NTS1 in cultured cells and tissue sections by immunohistochemistry.

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Alternative Names NTS1, NTR1, Neurotensin Receptor Type 1, Neurotensin... more

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Alternative Names	NTS1, NTR1, Neurotensin Receptor Type 1, Neurotensin Receptor 1
IUPHAR Target ID	309
UniProt ID	P30989
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	-
Species Reactivity	Human
Host / Isotype	Guinea Pig / IgG
Class	Polyclonal
Immunogen	A synthetic peptide presents part of carboxyl-terminal tail of human NTS1.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Immunohistochemical identification of... more

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Figure 1. Immunohistochemical identification of Neurotensin Receptor 1 in Accumbens. Sections were dewaxed, microwaved in citric acid, and incubated with anti-Neurotensin Receptor 1 (NTS1) antibody (7TM0309N-ICGP) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-guinea pig IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin.

Figure 1. Validation of the Neurotensin Receptor 1 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Neurotensin Receptor 1 (NTS1) were lysed and immunoblotted with the anti-NTS1 antibody (7TM0309N-ICGP) at a dilution of 1:1000.