pT338/pS341-LPA1 (phospho-Lysophosphatidic Acid Receptor 1 Antibody)

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Agonist-induced Threonine338/Serine341 phosphorylation of the Lysophosphatidic Acid Receptor 1

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Order number: 7TM0272A
Content: 100 µl
Host: Rabbit

Threonine338/Serine341 (T338/S341) is major phosphorylation site of the Lysophosphatidic... more

Threonine338/Serine341 (T338/S341) is major phosphorylation site of the Lysophosphatidic Acid Receptor 1 (LPA1). The pT338/pS341-LPA1 antibody detects phosphorylation in response to agonists. T338/S341 phosphorylation is likely to be involved in efficient ligand sequestration by LPA.

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Alternative Names Edg-2, endothelial differentiation G protein-coupled... more

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Alternative Names	Edg-2, endothelial differentiation G protein-coupled receptor 2 Receptor, GPR26, VZG1
IUPHAR Target ID	272
UniProt ID	Q92633
Western Blot (WB)	1:1000
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human LPA1 around the phosphorylation site of Thr338/Ser341.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Threonine338/Serine341 phosphorylation of the Lysophosphatidic Acid... more

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Figure 1. Agonist-induced Threonine338/Serine341 phosphorylation of the Lysophosphatidic Acid Receptor 1. Upper panel, HEK293 cells stably expressing the LPA1-Receptor (LPA1) were either not exposed or exposed to 1 µM of LPA1-Receptor agonists 1-oleoyl-LPA for 30 minutes. Cells were lysed and immunoblotted with the anti-pT338/pS341-LPA1 antibody (7TM0272A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-LPA1 antibody to confirm equal loading of the gel.

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