pS368-KISS1R (phospho-Kisspeptin Receptor Antibody)

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Agonist-induced Serine368 phosphorylation of the Kisspeptin Receptor

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Order number: 7TM0266A
Content: 100 µl
Host: Rabbit

Serine368 (S368) is major phosphorylation site of the Kisspeptin Receptor (KISS1R).... more

Serine368 (S368) is major phosphorylation site of the Kisspeptin Receptor (KISS1R). The pS368-KISS1R antibody detects phosphorylation in response to agonists. S368 phosphorylation is likely to be involved in efficient ligand sequestration by Kisspeptin.

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Alternative Names Metastin Receptor, KiSS1-derived peptide Receptor, GPR54... more

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Alternative Names	Metastin Receptor, KiSS1-derived peptide Receptor, GPR54
IUPHAR Target ID	266
UniProt ID	Q969F8
Western Blot (WB)	1:1000
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human KISS1R around the phosphorylation site of Ser368.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Serine368 phosphorylation of the Kisspeptin Receptor. Upper panel,... more

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Figure 1. Agonist-induced Serine368 phosphorylation of the Kisspeptin Receptor. Upper panel, HEK293 cells stably expressing the Kisspeptin receptor (KISS1R) were either not exposed or exposed to 100 nM Kisspeptin-13 for 30 minutes. Cells were lysed and immunoblotted with the anti-pS368-KISS1R antibody (7TM0266A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with a phosphorylation-independent anti-KISS1R antibody to confirm equal loading of the gel.

Be the first to analyze the ACKR2 phosphorylation using our anti-pS348/pS350-ACKR2... more

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Be the first to analyze the ACKR2 phosphorylation using our anti-pS348/pS350-ACKR2 antibody and let us know.

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Download 7TM Western Blot Protocol

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