NPSR (IHC-grade), Neuropeptide S Receptor Antibody

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Preview: Immunohistochemical identification of NPSR in Duodenum

Preview: Validation of the NPSR in transfected HEK293 cells

Immunohistochemical identification of NPSR in Duodenum

Validation of the NPSR in transfected HEK293 cells

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Order number: 7TM0302N-IC
Content: 100 µl
Host: Rabbit

The NPSR antibody is directed against the distal end of the carboxyl-terminal tail of human... more

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The NPSR antibody is directed against the distal end of the carboxyl-terminal tail of human Neuropeptide S Receptor. It can be used to detect total NPS receptors in Western blots independent of phosphorylation. The NPSR antibody can also be used to isolate and enrich NPS receptors from cell and tissue lysates. It also detects NPSR in cultured cells and tissue sections by immunohistochemistry.

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Alternative Names VRR1, Vasopressin Receptor-related Receptor IUPHAR... more

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Alternative Names	VRR1, Vasopressin Receptor-related Receptor
IUPHAR Target ID	302
UniProt ID	Q6W5P4
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide presents distal end of carboxy-terminal of human NPSR.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Immunohistochemical identification of NPSR in Duodenum. Sections... more

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Figure 1. Immunohistochemical identification of NPSR in Duodenum. Sections were dewaxed, microwaved in citric acid, and incubated with anti-NPSR (NPS Receptor) antibody (7TM0302N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin.

Figure 2. Validation of the NPSR in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the NPS Receptor (NPSR) were lysed and immunoblotted with the phosphorylation-independent anti-NPSR antibody (7TM0302N-IC) at a dilution of 1:1000.