β2 (non-phospho), β2-Adrenoceptor Antibody

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Validation of the β2-Adrenoceptor in transfected HEK293 cells

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Order number: 7TM0029N
Content: 100 µl
Host: Rabbit

The non-phospho- β2 antibody is directed against the third intracellular loop of human β2 . It... more

The non-phospho-β2 antibody is directed against the third intracellular loop of human β2. It can be used to detect total β2 receptors in Western blots independent of phosphorylation. The non-phospho-β2 antibody can also be used to isolate and enrich β2 receptors from cell and tissue lysates.

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Alternative Names β2,B2AR, ADRB2R, β2 Adrenergic... more

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Alternative Names	β2,B2AR, ADRB2R, β2 Adrenergic Receptor, β2-Adrenoceptor
IUPHAR Target ID	29
UniProt ID	P07550
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	-
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide derived from human β2 with the sequence GLRRSSKF.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Validation of the β2-Adrenoceptor in transfected HEK293 cells. Native... more

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Figure 1. Validation of the β2-Adrenoceptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the β2-Adrenoceptor (β2) were lysed and immunoblotted with the phosphorylation-independent anti-β2 antibody (7TM0029N-WB) at a dilution of 1:1000.