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- Order number: 7TM0057A
- Content: 100 µl
- Host: Rabbit
Serine335/Serine336 (S335/S336) is major phosphorylation site of the Cannabinoid Receptor 2 (CB2). The pS335/pS336-CB2 antibody detects phosphorylation in response to agonists. S335/S336 phosphorylation is likely to be involved in efficient ligand sequestration by CB2.
Alternative Names | CB2-R, Peripheral Cannabinoid Receptor |
IUPHAR Target ID | 57 |
UniProt ID | P34927 |
Western Blot (WB) | 1:1000 |
Species Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Immunogen | A synthetic phosphopeptide derived from human CB2 around the phosphorylation site of Ser335/Ser336. |
Form | Liquid |
Purification | Antigen affinity chromatography |
Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Agonist-induced Serine335/Serine336 phosphorylation of the Cannabinoid Receptor 2. Upper panel, HEK293 cells stably expressing the CB2-Receptor (CB2) were either not exposed or exposed to 1 µM of CB2-Receptor agonists CP55940 or WIN55212-2 for 30 minutes. Cells were lysed and immunoblotted with the anti-pS335/pS336-CB2 antibody (7TM0057A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-CB2 antibody to confirm equal loading of the gel.
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