pS286/pT287/pS288-M2 (phospho-M2 Muscarinic Acetylcholine Receptor Antibody)

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Agonist-induced Serine286/Threonine287/Serine288 phosphorylation of the M2 Muscarinic Acetycholine Receptor

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Order number: 7TM0014C
Content: 100 µl
Host: Rabbit

Serine286/Threonine287/Serine288 is a major phosphorylation site of the M2 Muscarinic... more

Serine286/Threonine287/Serine288 is a major phosphorylation site of the M2 Muscarinic Acetylcholine Receptor. The pS286/pT287/pS288-M2 antibody detects phosphorylation in response to high-efficacy agonists but not after PKC activation. S286/T287/S288 phosphorylation is a key regulator of M2 desensitization, β-arrestin recruitment and internalization.

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Alternative Names AChR M2, Chrm 2, Cholinergic Receptor muscarinic 2... more

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Alternative Names	AChR M2, Chrm 2, Cholinergic Receptor muscarinic 2
IUPHAR Target ID	14
UniProt ID	P08172
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	-
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human M2 around the phosphorylation site of Ser286/Thr287/Ser288.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Serine286/Threonine287/Serine288 phosphorylation of the M2... more

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Figure 1. Agonist-induced Serine286/Threonine287/Serine288 phosphorylation of the M2 Muscarinic Acetycholine Receptor. Upper panel, HEK293 cells stably expressing the 5-Hydroxytryptamine Receptor 4 (M2) were either not exposed or exposed to 100 µM carbachol for 30 minutes. Cells were lysed and immunoblotted with the anti-pS286/pT287/pS288-M2 antibody (7TM0014C) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-M2 antibody to confirm equal loading of the gel.

Be the first to decipher the M2 phosphorylation barcode and let us know. more

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Be the first to decipher the M2 phosphorylation barcode and let us know.

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