M2 (non-phospho-M2 Muscarinic Acetylcholine Receptor Antibody)

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Validation of the M2 Muscarinic Acetylcholine Receptor in transfected HEK293 cells

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Order number: 7TM0014N
Content: 100 µl
Host: Rabbit

The non-phospho-M2 receptor antibody is directed against the 3rd loop of human M2 Muscarinic... more

The non-phospho-M2 receptor antibody is directed against the 3rd loop of human M2 Muscarinic Acetylcholine Receptor (M2). It can be used to detect total M2 receptors in Western blots independent of phosphorylation. The non-phospho-M2 antibody can also be used to isolate and enrich M2 receptors from cell and tissue lysates.

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Alternative Names AChR M2, Chrm 2, Cholinergic Receptor muscarinic 2... more

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Alternative Names	AChR M2, Chrm 2, Cholinergic Receptor muscarinic 2
IUPHAR Target ID	14
UniProt ID	P08172
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	-
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide presents the third intracellular loop of human M2.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Validation of the M2 Muscarinic Acetylcholine Receptor in transfected HEK293 cells.... more

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Figure 1. Validation of the M2 Muscarinic Acetylcholine Receptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the M2 Muscarinic Acetylcholine Receptor (M2) were lysed and immunoblotted with the phosphorylation-independent anti-M2 antibody (7TM0014N) at a dilution of 1:1000.

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