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- Order number: 7TM0300B
- Content: 100 µl
- Host: Rabbit
Serine385/Serine387 (S385/S387) is major phosphorylation site of the Neuropeptide FF Receptor 1 (NPFF1). The pS385/pS387-NPFF1 antibody detects phosphorylation in response to agonists. S385/S387 phosphorylation is likely to be involved in efficient ligand sequestration by NPFF1.
Alternative Names | NPFF1R1, RF amide-related Peptide Receptor OT7T022 |
IUPHAR Target ID | 300 |
UniProt ID | Q9GZQ6 |
Western Blot (WB) | 1:1000 |
Species Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Immunogen | A synthetic phosphopeptide derived from human NPFF1 around the phosphorylation site of Ser385/Ser387. |
Form | Liquid |
Purification | Antigen affinity chromatography |
Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Agonist-induced Serine385/Serine387 phosphorylation of the Neuropeptide Receptor 1. Upper panel, HEK293 cells stably expressing the Neuropeptide FF receptor 1 (NPFF1) were either not exposed or exposed to 100 nM NPFF for 30 minutes. Cells were lysed and immunoblotted with the anti-pS385/pS387-NPFF1 antibody (7TM0300B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-NPFF1 antibody to confirm equal loading of the gel.
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